ArticleStem cells translational medicine2017
MMP-2 and MMP-14 Silencing Inhibits VEGFR2 Cleavage and Induces the Differentiation of Porcine Adipose-Derived Mesenchymal Stem Cells to Endothelial Cells.
Article in Stem cells translational medicine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 30 citations in OpenAlex.
- Progressing Regenerative Medicine: Integrating Bioprinting Platforms for Stem Cell Applications.Stem cells international · 2026Review
- Effects of dengue virus type-2 serotype (DENV-2) on the expression profile of matrix metalloproteinases in THP-1, monocyte cells and their roles in endothelial dysfunctions: protective effect of atorvastatin.Frontiers in cellular and infection microbiology · 2025Article
- The Application of Stem Cells and Exosomes in Promoting Nerve Conduits for Peripheral Nerve Repair.Biomaterials research · 2025Review
- Adipose-Derived Stem Cells to Treat Ischemic Diseases: The Case of Peripheral Artery Disease.International journal of molecular sciences · 2023Review
- Adipose-derived mesenchymal stem cells and wound healing: Potential clinical applications in wound repair.Saudi medical journal · 2022Article
- Stem Cell Therapies for Chronic Liver Diseases: Progress and Challenges.Stem cells translational medicine · 2022Review
- [Clinical research in cell therapy of liver diseases: progress and challenges].Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology · 2022Review
- Abnormal Proteomics Profile of Plasma Reveals the Immunological Pathogenesis of Severe Aplastic Anemia.Disease markers · 2022Article
- Angiogenic Effects and Crosstalk of Adipose-Derived Mesenchymal Stem/Stromal Cells and Their Extracellular Vesicles with Endothelial Cells.International journal of molecular sciences · 2021Review
- Effects of quercetin on cell differentiation and adipogenesis in 3T3-L1 adipocytes.Nutrition research and practice · 2021Article
- Analyses of soluble endoglin and matrix metalloproteinase 14 using enzyme-linked immunosorbent assay in the diagnosis and assessment of severity of early- and late-onset pre-eclampsiaJournal of the Turkish German Gynecological Association · 2021Article
- Role of the CXCR4-SDF1-HMGB1 pathway in the directional migration of cells and regeneration of affected organs.World journal of stem cells · 2020Review
- [Research progress of adipose-derived stem cells in promoting the repair of peripheral nerve injury].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2020Review
- Derivation of endothelial cells from porcine induced pluripotent stem cells by optimized single layer culture system.Journal of veterinary science · 2020Article
- Review
- Injectable and protease-degradable hydrogel for siRNA sequestration and triggered delivery to the heart.Journal of controlled release : official journal of the Controlled Release Society · 2018Article
- Expression of MMP-2 and TIMP-3 with incidence and prognosis of giant-cell tumor of the bone.Oncology letters · 2018Article
- ERK signaling is required for VEGF-A/VEGFR2-induced differentiation of porcine adipose-derived mesenchymal stem cells into endothelial cells.Stem cell research & therapy · 2017Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The molecular mechanisms that control the ability of adipose-derived mesenchymal stem cells (AMSCs) to remodel three-dimensional extracellular matrix barriers during differentiation are not clearly understood. Herein, we studied the expression of matrix metalloproteinases (MMPs) during the differentiation of AMSCs to endothelial cells (ECs) in vitro. MSCs were isolated from porcine abdominal adipose tissue, and characterized by immunopositivity to CD44, CD90, CD105, and immunonegativity to CD14 and CD45. Plasticity of AMSCs was confirmed by multilineage differentiation. The mRNA transcripts for MMPs and Tissue Inhibitor of Metalloproteinases (TIMPs), and protein expression of EC markers were analyzed. The enzyme activity and protein expression were analyzed by gelatin zymography, enzyme-linked immunosorbent assay (ELISA), and Western blot. The differentiation of AMSCs to ECs was confirmed by mRNA and protein expressions of the endothelial markers. The mRNA transcripts for MMP-2 and MMP-14 were significantly increased during the differentiation of MSCs into ECs. Findings revealed an elevated MMP-14 and MMP-2 expression, and MMP2 enzyme activity. Silencing of MMP-2 and MMP-14 significantly increased the expression of EC markers, formation of capillary tubes, and acetylated-low-density lipoprotein uptake, and decreased the cleavage of vascular endothelial growth factor receptor type 2 (VEGFR2). Inhibition of VEGFR2 significantly decreased the expression of EC markers. These novel findings demonstrate that the upregulation of MMP2 and MMP14 has an inhibitory effect on the differentiation of AMSCs to ECs, and silencing these MMPs inhibit the cleavage of VEGFR2 and stimulate the differentiation of AMSCs to ECs. These findings provide a potential mechanism for the regulatory role of MMP-2 and MMP-14 in the re-endothelialization of coronary arteries following intervention. Stem Cells Translational Medicine 2017;6:1385-1398.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.