ArticleStem cells translational medicine2017
Highly Efficient Neural Conversion of Human Pluripotent Stem Cells in Adherent and Animal-Free Conditions.
Article in Stem cells translational medicine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Electrical stimulation and stem cell subdural implantation decrease microglia reactivity after spinal cord injury.Materials today. Bio · 2026Article
- Fetal bovine serum: how to leave it behind in the pursuit of more reliable science.Frontiers in toxicology · 2025Review
- Neural Differentiation of Induced Pluripotent Stem Cells for a Xenogeneic Material-Free 3D Neurological Disease Model Neurulation from Pluripotent Cells Using a Human Hydrogel.Current issues in molecular biology · 2023Article
- Stable and efficient generation of functional iPSC-derived neural progenitor cell rosettes through regulation of collective cell-cell behavior.Frontiers in bioengineering and biotechnology · 2023Article
- Recovery of Human Embryonic Stem Cells-Derived Neural Progenitors Exposed to Hypoxic-Ischemic-Reperfusion Injury by Indirect Exposure to Wharton's Jelly Mesenchymal Stem Cells Through Phosphatidyl-inositol-3-Kinase Pathway.Cellular and molecular neurobiology · 2022Article
- The roles and applications of neural stem cells in spinal cord injury repair.Frontiers in bioengineering and biotechnology · 2022Review
- Efficient induction of neural progenitor cells from human ESC/iPSCs on Type I Collagen.Science China. Life sciences · 2021Article
- Human-Induced Neural and Mesenchymal Stem Cell Therapy Combined with a Curcumin Nanoconjugate as a Spinal Cord Injury Treatment.International journal of molecular sciences · 2021Article
- Activation of Neurogenesis in Multipotent Stem Cells Cultured In Vitro and in the Spinal Cord Tissue After Severe Injury by Inhibition of Glycogen Synthase Kinase-3.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2021Article
- Multiplexed analysis of neural cytokine signaling by a novel neural cell-cell interaction microchip.Lab on a chip · 2020Article
- Effect of Cell Spreading on Rosette Formation by Human Pluripotent Stem Cell-Derived Neural Progenitor Cells.Frontiers in cell and developmental biology · 2020Article
- Recent Progress in the Regeneration of Spinal Cord Injuries by Induced Pluripotent Stem Cells.International journal of molecular sciences · 2019Review
- A scalable solution for isolating human multipotent clinical-grade neural stem cells from ES precursors.Stem cell research & therapy · 2019Article
- Neural induction of porcine-induced pluripotent stem cells and further differentiation using glioblastoma-cultured medium.Journal of cellular and molecular medicine · 2019Article
- Article
- Restoring Motor Neurons in Spinal Cord Injury With Induced Pluripotent Stem Cells.Frontiers in cellular neuroscience · 2019Review
- Establishment of stable iPS-derived human neural stem cell lines suitable for cell therapies.Cell death & disease · 2018Article
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Authors and funding
15 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neural differentiation of human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) can produce a valuable and robust source of human neural cell subtypes, holding great promise for the study of neurogenesis and development, and for treating neurological diseases. However, current hESCs and hiPSCs neural differentiation protocols require either animal factors or embryoid body formation, which decreases efficiency and yield, and strongly limits medical applications. Here we develop a simple, animal-free protocol for neural conversion of both hESCs and hiPSCs in adherent culture conditions. A simple medium formula including insulin induces the direct conversion of >98% of hESCs and hiPSCs into expandable, transplantable, and functional neural progenitors with neural rosette characteristics. Further differentiation of neural progenitors into dopaminergic and spinal motoneurons as well as astrocytes and oligodendrocytes indicates that these neural progenitors retain responsiveness to instructive cues revealing the robust applicability of the protocol in the treatment of different neurodegenerative diseases. The fact that this protocol includes animal-free medium and human extracellular matrix components avoiding embryoid bodies makes this protocol suitable for the use in clinic. Stem Cells Translational Medicine 2017;6:1217-1226.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.