ArticleApplied and environmental microbiology2017
Effects of Gelling Agent and Extracellular Signaling Molecules on the Culturability of Marine Bacteria.
Article in Applied and environmental microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Variations in deep-sea microbial composition and assembly mechanisms under different culture strategies.Frontiers in microbiology · 2026Article
- Recent strategies and methodological advances for microbial natural product research in the post-genomics era.Archives of microbiology · 2025Review
- A diffusion-based integrative approach for culturing previously uncultured bacteria from marine sediments.Marine life science & technology · 2025Article
- Gelling and reducing agents are potential carbon and energy sources in culturing of anaerobic microorganisms.Applied and environmental microbiology · 2025Article
- Last Decade Insights in Exploiting Marine Microorganisms as Sources of New Bioactive Natural Products.Marine drugs · 2025Review
- Agar lot-specific inhibition in the plating efficiency of yeast spores and cells.G3 (Bethesda, Md.) · 2024Article
- Laboratory horror stories: Poison in the agars.bioRxiv : the preprint server for biology · 2024Article
- Investigating eukaryotic and prokaryotic diversity and functional potential in the cold and alkaline ikaite columns in Greenland.Frontiers in microbiology · 2024Article
- Marine Microbial Polysaccharides: An Untapped Resource for Biotechnological Applications.Marine drugs · 2023Review
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- Shedding light on the composition of extreme microbial dark matter: alternative approaches for culturing extremophiles.Frontiers in microbiology · 2023Review
- Review
- Microbial diversity in tropical marine sediments assessed using culture-dependent and culture-independent techniques.Environmental microbiology · 2021Article
- Comparative Analysis of the Cultured and Total Bacterial Community in the Wheat Rhizosphere Microbiome Using Culture-Dependent and Culture-Independent Approaches.Microbiology spectrum · 2021Article
- Characterization of Environmental and Cultivable Antibiotic-Resistant Microbial Communities Associated with Wastewater Treatment.Antibiotics (Basel, Switzerland) · 2021Article
- Pseudomonas pratensis sp. nov., Isolated from Grassland Soil from Inner Mongolia, China.Current microbiology · 2021Review
- Triggering Growth via Growth Initiation Factors in Nature: A Putative Mechanism forFrontiers in microbiology · 2021Article
- Bacteria Cultivated From Sponges and Bacteria Not Yet Cultivated From Sponges-A Review.Frontiers in microbiology · 2021Review
- Marine Sediments Hold an Untapped Potential for Novel Taxonomic and Bioactive Bacterial Diversity.mSystems · 2020Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Only 1% of marine bacteria are currently culturable using standard laboratory procedures, and this is a major obstacle for our understanding of the biology of marine microorganisms and for the discovery of novel microbial natural products. Therefore, the purpose of this study was to investigate if improved cultivation conditions, including the use of an alternative gelling agent and supplementation with signaling molecules, improve the culturability of bacteria from seawater. Replacing agar with gellan gum improved viable counts 3- to 40-fold, depending on medium composition and incubation conditions, with a maximum of 6.6% culturability relative to direct cell counts. Through V4 amplicon sequencing we found that culturable diversity was also affected by a change in gelling agent, facilitating the growth of orders not culturable on agar-based substrates. Community analyses showed that communities grown on gellan gum substrates were significantly different from communities grown on agar and that they covered a larger fraction of the seawater community. Other factors, such as incubation temperature and time, had less obvious effects on viable counts and culturable diversity. Supplementation with acylated homoserine lactones (AHLs) did not have a positive effect on total viable counts or a strong effect on culturable diversity. However, low concentrations of AHLs increased the relative abundance of sphingobacteria. Hence, with alternative growth substrates, it is possible to significantly increase the number and diversity of cultured marine bacteria.
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