ArticleBMC microbiology2017
Porphyromonas gingivalis can invade periodontal ligament stem cells.
Article in BMC microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Inhibition of miR-508-5p Promotes Osteogenic-Angiogenic Coupling to Enhance Bone Formation.International journal of molecular sciences · 2026Article
- P. gingivalis-host interactions direct antibiotic adjuvants for periodontitis antimicrobial therapy.International journal of oral science · 2026Article
- Article
- Hepatocyte Growth Factor Differentially Modulates Oral Microbiota in Early vs. Late Experimental Periodontitis.Biology · 2025Article
- Internalization and Colocalization of a Polyclonal Antibody Against Porphyromonas gingivalis FimA type I in Infected Cells.European journal of dentistry · 2025Article
- Identification and Validation of Aging- and Endoplasmic Reticulum Stress-Related Genes in Periodontitis Using a Competing Endogenous RNA Network.Inflammation · 2025Article
- Exploring heme and iron acquisition strategies of Porphyromonas gingivalis-current facts and hypotheses.FEMS microbiology reviews · 2025Review
- Hallmarks of Periodontitis.Current medicinal chemistry · 2025Review
- The Genetic Aspects of Periodontitis Pathogenesis and the Regenerative Properties of Stem Cells.Cells · 2024Review
- Periodontitis Continuum: Antecedents, Triggers, Mediators, and Treatment Strategies.Current medicinal chemistry · 2024Review
- Stem cell microencapsulation maintains stemness in inflammatory microenvironment.International journal of oral science · 2022Article
- Porphyromonas gingivalis outer membrane vesicles inhibit the invasion of Fusobacterium nucleatum into oral epithelial cells by downregulating FadA and FomA.Journal of periodontology · 2022Article
- Superparamagnetic Iron Oxide Nanoparticles Protect Human Gingival Fibroblasts fromInternational journal of nanomedicine · 2022Article
- Quercetin Prevents Oxidative Stress-Induced Injury of Periodontal Ligament Cells and Alveolar Bone Loss in Periodontitis.Drug design, development and therapy · 2021Article
- Investigation of arginine A-specific cysteine proteinase gene expression profiling in clinical Porphyromonas gingivalis isolates against photokilling action of the photo-activated disinfection.Lasers in medical science · 2018Article
- Crosstalk between salivary citrullinated proteins and oral health status in patients with rheumatoid arthritis: A cross-sectional study.Journal of oral and maxillofacial pathology : JOMFPArticle
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPorphyromonas gingivalis is strongly associated with the development, progression, severity and recurrence of periodontitis. Periodontal ligament stem cells (PDLSCs) play an important role in the maintenance of periodontal tissue self-renewal and repair. The purpose of this study was to investigate the ability of P. gingivalis to infect PDLSCs using an in vitro monolayer model.
methodsWe separated and cultured primary PDLSCs using the tissue block with limiting dilution method. The efficiency of P. gingivalis (ATCC 33277) infection of PDLSCs was measured using agar plate culture and quantitative polymerase chain reaction (q-PCR) methods. PDLSCs infected with P. gingivalis were also observed by transmission electron microscopy.
resultsWe assessed stem cell properties including cell morphology, clone formation, growth activity, cell surface antigens and multiple differentiation capacity. The infection rates of P. gingivalis in PDLSC at MOIs of 50, 100, 200, and 500 were 5.83%, 8.12%, 7.77% and 7.53% according to the agar plate culture method. By q-PCR, the efficiencies of P. gingivalis infection of PDLSCs at MOIs of 50, 100, 200, and 500 were 6.74%, 10.56%, 10.36% and 9.78%, respectively. Overall, the infection efficiency based on q-PCR was higher than that according to agar plate culture. Using transmission electron microscopy, we verified that P. gingivalis (ATCC 33277) could infect and invade PDLSCs after 2 h of incubation, and endocytic vacuoles were not found surrounding the internalized bacteria.
conclusionsIn conclusion, our data demonstrate that P. gingivalis can invade PDLSCs.
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