ArticleScientific reports2017
High-throughput in situ cell electroporation microsystem for parallel delivery of single guide RNAs into mammalian cells.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- Liquid Metal Nanotransformers for Drug-Resistant Pan-Cancer Therapy in Patient-Derived Organoids.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- High-Throughput Microfluidic Electroporation (HTME): A Scalable, 384-Well Platform for Multiplexed Cell Engineering.Bioengineering (Basel, Switzerland) · 2025Article
- Development of Specialized Microelectrode Arrays with Local Electroporation Functionality.Annals of biomedical engineering · 2024Article
- A robot-assisted acoustofluidic end effector.Nature communications · 2022Article
- Recent Advances in Microscale Electroporation.Chemical reviews · 2022Review
- Grouped-seq for integrated phenotypic and transcriptomic screening of patient-derived tumor organoids.Nucleic acids research · 2022Article
- Tailoring Materials with Specific Wettability in Biomedical Engineering.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Review
- In situ electroporation of mammalian cells through SiOScientific reports · 2021Article
- CRISPR-Associated (CAS) Effectors Delivery via Microfluidic Cell-Deformation Chip.Materials (Basel, Switzerland) · 2021Review
- Lung cancer organoids analyzed on microwell arrays predict drug responses of patients within a week.Nature communications · 2021Article
- A Novel White-to-Blue Colony Formation Assay to Select for Optimized sgRNAs.Molecular biotechnology · 2021Article
- Recent Progress in Wearable Biosensors: From Healthcare Monitoring to Sports Analytics.Biosensors · 2020Review
- Metallic Nanoparticle-Based Optical Cell Chip for Nondestructive Monitoring of Intra/Extracellular Signals.Pharmaceutics · 2020Review
- A review of emerging physical transfection methods for CRISPR/Cas9-mediated gene editing.Theranostics · 2020Review
- 3D-printing enabled micro-assembly of a microfluidic electroporation system for 3D tissue engineering.Lab on a chip · 2019Article
- High-Throughput Platform for Efficient Chemical Transfection, Virus Packaging, and Transduction.Micromachines · 2019Article
- Nanoliter Centrifugal Liquid Dispenser Coupled with Superhydrophobic Microwell Array Chips for High-Throughput Cell Assays.Micromachines · 2018Article
- Enhanced gene delivery in tumor cells using chemical carriers and mechanical loadings.PloS one · 2018Article
- Label-free and real-time monitoring of single cell attachment on template-stripped plasmonic nano-holes.Scientific reports · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Arrayed genetic screens mediated by the CRISPR/Cas9 technology with single guide RNA (sgRNA) libraries demand a high-throughput platform capable of transfecting diverse cell types at a high efficiency in a genome-wide scale for detection and analysis of sophisticated cellular phenotypes. Here we developed a high-throughput in situ cell electroporation (HiCEP) microsystem which leveraged the superhydrophobic feature of the microwell array to achieve individually controlled conditions in each microwell and coupled an interdigital electrode array chip with the microwells in a modular-based scheme for highly efficient delivery of exogenous molecules into cells. Two plasmids encoding enhanced green and red fluorescent proteins (EGFP and ERFP), respectively, were successfully electroporated into attached HeLa cells on a 169-microwell array chip with transfection efficiencies of 71.6 ± 11.4% and 62.9 ± 2.7%, and a cell viability above 95%. We also successfully conducted selective electroporation of sgRNA into 293T cells expressing the Cas9 nuclease in a high-throughput manner and observed the four-fold increase of the GFP intensities due to the repair of the protein coding sequences mediated by the CRISPR/Cas9 system. This study proved that this HiCEP system has the great potential to be used for arrayed functional screens with genome-wide CRISPR libraries on hard-to-transfect cells in the future.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.