ArticleThe ISME journal2017
Host specificity of Symbiodinium variants revealed by an ITS2 metahaplotype approach.
Article in The ISME journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Biogeographical Patterns of Coral-Associated Symbiodiniaceae Across Thermal Regimes Including Trace Molecular Detection ofEcology and evolution · 2026Article
- Molecular identification and studies on genetic diversity and structure-related GC heterogeneity of Spatholobus Suberectus based on ITS2.Scientific reports · 2024Article
- Chemical mutagenesis and thermal selection of coral photosymbionts induce adaptation to heat stress with trait trade-offs.Evolutionary applications · 2023Article
- Micro and macroevolution of sea anemone venom phenotype.Nature communications · 2023Article
- Building consensus around the assessment and interpretation of Symbiodiniaceae diversity.PeerJ · 2023Article
- Signatures of selection underpinning rapid coral adaptation to the world's warmest reefs.Science advances · 2022Article
- Article
- Distribution and biogeography ofEcology and evolution · 2020Article
- A framework for in situ molecular characterization of coral holobionts using nanopore sequencing.Scientific reports · 2020Article
- Homogenization of Endosymbiont Communities Hosted by Equatorial Corals during the 2016 Mass Bleaching Event.Microorganisms · 2020Article
- Uncovering the role of Symbiodiniaceae assemblage composition and abundance in coral bleaching response by minimizing sampling and evolutionary biases.BMC microbiology · 2020Article
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- Article
- Article
- Genome Size, rDNA Copy, and qPCR Assays for Symbiodiniaceae.Frontiers in microbiology · 2020Article
- Depth Influences Symbiodiniaceae Associations AmongFrontiers in microbiology · 2020Article
- Considerations for maximizing the adaptive potential of restored coral populations in the western Atlantic.Ecological applications : a publication of the Ecological Society of America · 2019Article
- Transgenerational inheritance of shuffled symbiont communities in the coral Montipora digitata.Scientific reports · 2019Article
- SymPortal: A novel analytical framework and platform for coral algal symbiont next-generation sequencing ITS2 profiling.Molecular ecology resources · 2019Article
- Coral microbiome diversity reflects mass coral bleaching susceptibility during the 2016 El Niño heat wave.Ecology and evolution · 2019Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Analysis of the widely used ITS region is confounded by the presence of intragenomic variants (IGVs). In Symbiodinium, the algal symbionts of reef building corals, deep-sequencing analyses are used to characterise communities within corals, yet these analyses largely overlook IGVs. Here we consider that distinct ITS2 sequences could represent IGVs rather than distinct symbiont types and argue that symbionts can be distinguished by their proportional composition of IGVs, described as their ITS2 metahaplotype. Using our metahaplotype approach on Minimum Entropy Decomposition (MED) analysis of ITS2 sequences from the corals Acropora downingi, Cyphastrea microphthalma and Playgyra daedalea, we show the dominance of a single species-specific Symbiodinium C3 variant within each coral species. We confirm the presence of these species-specific symbionts using the psbA non-coding region. Our findings highlight the importance of accounting for IGVs in ITS2 analyses and demonstrate their capacity to resolve biological patterns that would otherwise be overlooked.
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