Evidence map›Paper›PMID 28211677›Full record

ReviewChemical reviews2017

Super-Resolution Microscopy: Shedding Light on the Cellular Plasma Membrane.

Matthew B Stone, Sarah A Shelby, Sarah L Veatch

Abstract readReview
In one paragraph

Review in Chemical reviews, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.

0numbers the graph read from it
0cells of the map it votes in
59citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

59 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Matthew B StoneDepartment of Biophysics, University of Michigan , 930 North University Avenue, Ann Arbor, Michigan 48109, United States.
Sarah A ShelbyDepartment of Biophysics, University of Michigan , 930 North University Avenue, Ann Arbor, Michigan 48109, United States.
Sarah L VeatchDepartment of Biophysics, University of Michigan , 930 North University Avenue, Ann Arbor, Michigan 48109, United States.ORCID http://orcid.org/0000-0002-9317-2308

Funding

Ion channel regulation by heterogeneous membranesR01GM110052 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI VEATCH, SARAH L · 2014 to 2022
$2.9M
NIGMS NIH HHS R01 GM110052
6 · The paper itself

Abstract

Lipids and the membranes they form are fundamental building blocks of cellular life, and their geometry and chemical properties distinguish membranes from other cellular environments. Collective processes occurring within membranes strongly impact cellular behavior and biochemistry, and understanding these processes presents unique challenges due to the often complex and myriad interactions between membrane components. Super-resolution microscopy offers a significant gain in resolution over traditional optical microscopy, enabling the localization of individual molecules even in densely labeled samples and in cellular and tissue environments. These microscopy techniques have been used to examine the organization and dynamics of plasma membrane components, providing insight into the fundamental interactions that determine membrane functions. Here, we broadly introduce the structure and organization of the mammalian plasma membrane and review recent applications of super-resolution microscopy to the study of membranes. We then highlight some inherent challenges faced when using super-resolution microscopy to study membranes, and we discuss recent technical advancements that promise further improvements to super-resolution microscopy and its application to the plasma membrane.

Indexed as

Cell MembraneLightAnimalsHumansMicroscopy

Identifiers

PMID28211677
PMCPMC5471115

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.