ArticleScientific reports2017
High-resolution 3D imaging of whole organ after clearing: taking a new look at the zebrafish testis.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Multiphoton Luminescence Imaging: A Label-Free Tool for Visualizing the Long-Term Fate of Gold Nanoparticles in Tissue.ACS nano · 2025Article
- The physical roles of different posterior tissues in zebrafish axis elongation.Nature communications · 2025Article
- T-CLEARE: a pilot community-driven tissue clearing protocol repository.Frontiers in bioengineering and biotechnology · 2024Article
- TSA-PACT: a method for tissue clearing and immunofluorescence staining on zebrafish brain with improved sensitivity, specificity and stability.Cell & bioscience · 2023Article
- Challenges and advances in optical 3D mesoscale imaging.Journal of microscopy · 2022Article
- Transgenic fluorescent zebrafish lines that have revolutionized biomedical research.Laboratory animal research · 2021Review
- The frontier of live tissue imaging across space and time.Cell stem cell · 2021Review
- Three-Dimensional Imaging in Stem Cell-Based Researches.Frontiers in veterinary science · 2021Review
- Tissue Optical Clearing for Biomedical Imaging: From In Vitro to In Vivo.Advances in experimental medicine and biology · 2021Article
- Looking through Brains with Fast Passive CLARITY: Zebrafish, Rodents, Non-human Primates and Humans.Bio-protocol · 2019Article
- FACT or PACT: A Comparison between Free-Acrylamide and Acrylamide-Based Passive Sodium Dodecyl Sulfate Tissue Clearing for whole Tissue Imaging.Cell journal · 2019Article
- Imaging the brain in 3D using a combination of CUBIC and immunofluorescence staining.Biomedical optics express · 2019Article
- A cationic near infrared fluorescent agent and ethyl-cinnamate tissue clearing protocol for vascular staining and imaging.Scientific reports · 2019Article
- Skeletal Muscle CLARITY: A Preliminary Study of Imaging The Three-Dimensional Architecture of Blood Vessels and Neurons.Cell journal · 2018Article
- Evaluation of seven optical clearing methods in mouse brain.Neurophotonics · 2018Article
- Hydrogel-Tissue Chemistry: Principles and Applications.Annual review of biophysics · 2018Review
- Optimization of GFP Fluorescence Preservation by a Modified uDISCO Clearing Protocol.Frontiers in neuroanatomy · 2018Article
- zPACT: Tissue Clearing and Immunohistochemistry on Juvenile Zebrafish Brain.Bio-protocol · 2017Article
- Frontiers in Toxicogenomics in the Twenty-First Century-the Grand Challenge: To Understand How the Genome and Epigenome Interact with the Toxic Environment at the Single-Cell, Whole-Organism, and Multi-Generational Level.Frontiers in genetics · 2017Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Zebrafish testis has become a powerful model for reproductive biology of teleostean fishes and other vertebrates and encompasses multiple applications in applied and basic research. Many studies have focused on 2D images, which is time consuming and implies extrapolation of results. Three-dimensional imaging of whole organs recently became an important challenge to better understand their architecture and allow cell enumeration. Several protocols have thus been developed to enhance sample transparency, a limiting step for imaging large biological samples. However, none of these methods has been applied to the zebrafish testis. We tested five clearing protocols to determine if some of them could be applied with only small modifications to the testis. We compared clearing efficiency at both macroscopic and microscopic levels. CUBIC and PACT were suitable for an efficient transparency, an optimal optical penetration, the GFP fluorescence preservation and avoiding meaningful tissue deformation. Finally, we succeeded in whole testis 3D capture at a cellular resolution with both CUBIC and PACT, which will be valuable in a standard workflow to investigate the 3D architecture of the testis and its cellular content. This paves the way for further development of high content phenotyping studies in several fields including development, genetic or toxicology.
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