ArticleCongenital heart disease2017
Increased regurgitant flow causes endocardial cushion defects in an avian embryonic model of congenital heart disease.
Article in Congenital heart disease, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Clipping spline: interactive, dynamic 4D volume clipping and analysis based on thin plate spline.Biomedical optics express · 2025Article
- Segmentation of beating embryonic heart structures from 4-D OCT images using deep learning.Biomedical optics express · 2023Article
- Open-source, highly efficient, post-acquisition synchronization for 4D dual-contrast imaging of the mouse embryonic heart over development with optical coherence tomography.Biomedical optics express · 2023Article
- Automated endocardial cushion segmentation and cellularization quantification in developing hearts using optical coherence tomography.Biomedical optics express · 2022Article
- Following the Beat: Imaging the Valveless Pumping Function in the Early Embryonic Heart.Journal of cardiovascular development and disease · 2022Review
- Prenatal ethanol exposure impairs the conduction delay at the atrioventricular junction in the looping heart.American journal of physiology. Heart and circulatory physiology · 2021Article
- Folic acid prevents functional and structural heart defects induced by prenatal ethanol exposure.American journal of physiology. Heart and circulatory physiology · 2021Article
- Glutathione Protects the Developing Heart from Defects and Global DNA Hypomethylation Induced by Prenatal Alcohol Exposure.Alcoholism, clinical and experimental research · 2021Article
- Embryonic Mouse Cardiodynamic OCT Imaging.Journal of cardiovascular development and disease · 2020Review
- Semi-automated shear stress measurements in developing embryonic hearts.Biomedical optics express · 2020Article
- Live mechanistic assessment of localized cardiac pumping in mammalian tubular embryonic heart.Journal of biomedical optics · 2020Article
- Label-free optical imaging in developmental biology [Invited].Biomedical optics express · 2020Review
- Follow Me! A Tale of Avian Heart Development with Comparisons to Mammal Heart Development.Journal of cardiovascular development and disease · 2020Review
- Organ Dynamics and Hemodynamic of the Whole HH25 Avian Embryonic Heart, Revealed by Ultrasound Biomicroscopy, Boundary Tracking, and Flow Simulations.Scientific reports · 2019Article
- Optical coherence tomography forBiomedical optics express · 2019Article
- Hemodynamics in Cardiac Development.Journal of cardiovascular development and disease · 2018Article
- Complex regression Doppler optical coherence tomography.Journal of biomedical optics · 2018Article
- The avian embryo as a model for fetal alcohol spectrum disorder.Biochemistry and cell biology = Biochimie et biologie cellulaire · 2018Review
- Removing vessel constriction on the embryonic heart results in changes in valve gene expression, morphology, and hemodynamics.Developmental dynamics : an official publication of the American Association of Anatomists · 2018Article
- A review of optical pacing with infrared light.Journal of neural engineering · 2018Review
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10 authors.
Funding
Abstract
backgroundThe relationship between changes in endocardial cushion and resultant congenital heart diseases (CHD) has yet to be established. It has been shown that increased regurgitant flow early in embryonic heart development leads to endocardial cushion defects, but it remains unclear how abnormal endocardial cushions during the looping stages might affect the fully septated heart. The goal of this study was to reproducibly alter blood flow in vivo and then quantify the resultant effects on morphology of endocardial cushions in the looping heart and on CHDs in the septated heart.
methodsOptical pacing was applied to create regurgitant flow in embryonic hearts, and optical coherence tomography (OCT) was utilized to quantify regurgitation and morphology. Embryonic quail hearts were optically paced at 3 Hz (180 bpm, well above intrinsic rate 60-110 bpm) at stage 13 of development (3-4 weeks human) for 5 min. Pacing fatigued the heart and led to at least 1 h of increased regurgitant flow. Resultant morphological changes were quantified with OCT imaging at stage 19 (cardiac looping-4-5 weeks human) or stage 35 (4 chambered heart-8 weeks human).
resultsAll paced embryos imaged at stage 19 displayed structural changes in cardiac cushions. The amount of regurgitant flow immediately after pacing was inversely correlated with cardiac cushion size 24-h post pacing (P value < .01). The embryos with the most regurgitant flow and smallest cushions after pacing had a decreased survival rate at 8 days (P < .05), indicating that those most severe endocardial cushion defects were lethal. Of the embryos that survived to stage 35, 17/18 exhibited CHDs including valve defects, ventricular septal defects, hypoplastic ventricles, and common AV canal.
conclusionThe data illustrate a strong inverse relationship in which regurgitant flow precedes abnormal and smaller cardiac cushions, resulting in the development of CHDs.
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