ReviewFrontiers in microbiology2017
A Basic Guide to Real Time PCR in Microbial Diagnostics: Definitions, Parameters, and Everything.
Review in Frontiers in microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 387 papers, 3 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
387 citing papers in PubMed, 3 syntheses or guidelines pooled it.
- Viral Threats to Australian Fish and Prawns: Economic Impacts and Biosecurity Solutions-A Systematic Review.Viruses · 2025Pooled it
- Serum and urine nucleic acid screening tests for BK polyomavirus-associated nephropathy in kidney and kidney-pancreas transplant recipients.The Cochrane database of systematic reviews · 2024Pooled it
- Predicting the presence of infectious virus from PCR data: A meta-analysis of SARS-CoV-2 in non-human primates.PLoS pathogens · 2024Pooled it
- Comparative evaluation of two high-throughput qPCR systems for environmental and clinical microbiology applications.Applied and environmental microbiology · 2026Article
- Emerging Microbiological and Sensor-Based Approaches for Biofilm Detection in Meat and Poultry Processing Environments.Foods (Basel, Switzerland) · 2026Review
- Forensic Microbiomics and Trace Microbial Evidence: Molecular Innovations for Linking Suspects, Objects, and Environments.Microorganisms · 2026Review
- Wet Environmental Surveillance of Antimicrobial Resistance Genes in High-Risk Hospital Units.Antibiotics (Basel, Switzerland) · 2026Article
- Development and Validation of a Viability RT-qPCR Assay for Detecting Infectious Spring Viraemia of Carp Virus (SVCV).Journal of fish diseases · 2026Article
- Development of multiplex real-time RT‒PCR assays for the simultaneous detection and lineage differentiation of H9 avian influenza viruses.Poultry science · 2026Article
- Analytical Validation and Preliminary Diagnostic Performance Evaluation of GenoPATHX™ Multiplex qPCR for Quantitative Detection of KeyPathogens (Basel, Switzerland) · 2026Article
- Do Treponemes in Bovine Digital Dermatitis-Associated Claw Horn Lesions Preclude Co-Infection by Pyogenic Bacteria?Microorganisms · 2026Article
- Thermostable Bioluminescent Intercalating Dyes for Real-Time, Integrated Nucleic Acid Amplification and Detection.Angewandte Chemie (International ed. in English) · 2026Article
- Shedding light on bacterial fitness in a tug-of-war with liquid crystal emulsions.Nature communications · 2026Article
- Quantitative real-time PCR in the diagnosis of opportunistic infections in immunocompromised patients: Diagnostic performance, clinical utility, and future directions.Infectious diseases & immunity · 2026Review
- Biological risk assessment related to intracerebral injection of TMEV-DA.Archives of virology · 2026Article
- Robust detection in faeces andVeterinary and animal science · 2026Article
- SiOMikrochimica acta · 2026Article
- Comparative evaluation of Sm1-7-qPCR systems incorporated with Maxwell kit-based and NaOH-based DNA extraction methods for the detection of Schistosoma mansoni infection.Infectious diseases of poverty · 2026Article
- Development and comparative evaluation of TaqMan real-time PCR assays for detecting Enterocytozoon hepatopenaei (EHP) in penaeid shrimp.Scientific reports · 2026Article
- A knowledge-guided deep learning framework for quantitative nucleic acid testing.Briefings in bioinformatics · 2026Article
327 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Real time PCR (quantitative PCR, qPCR) is now a well-established method for the detection, quantification, and typing of different microbial agents in the areas of clinical and veterinary diagnostics and food safety. Although the concept of PCR is relatively simple, there are specific issues in qPCR that developers and users of this technology must bear in mind. These include the use of correct terminology and definitions, understanding of the principle of PCR, difficulties with interpretation and presentation of data, the limitations of qPCR in different areas of microbial diagnostics and parameters important for the description of qPCR performance. It is not our intention in this review to describe every single aspect of qPCR design, optimization, and validation; however, it is our hope that this basic guide will help to orient beginners and users of qPCR in the use of this powerful technique.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.