ArticleMolecular vision2017
Epac1 agonist decreased inflammatory proteins in retinal endothelial cells, and loss of Epac1 increased inflammatory proteins in the retinal vasculature of mice.
Article in Molecular vision, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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Who cites it
22 citing papers in PubMed, 25 citations in OpenAlex.
- Role of exchange proteins directly activated by cAMP signaling in vascular remodeling.Pharmacological reviews · 2025Review
- Ephrin B1 Regulates Inflammatory Pathways in Retinal Müller Cells.Journal of diabetes and clinical research · 2024Article
- Loss of cystatin C regulates permeability and inflammatory pathways in retina.Microvascular research · 2023Article
- Multiple Roles of cAMP in Vertebrate Retina.Cells · 2023Review
- Role of Takeda G protein‑coupled receptor 5 in microvascular endothelial cell dysfunction in diabetic retinopathy (Review).Experimental and therapeutic medicine · 2022Review
- TNFAIP3 may be key to TLR4-activation of the inflammasome in the retinal vasculature.Experimental eye research · 2022Article
- Epac1 and PKA regulate of P2X7 and NLRP3 inflammasome proteins in the retinal vasculature.Experimental eye research · 2022Article
- Prohibitin 1 Regulates Inflammatory Mediators and Reactive Oxygen Species in Retinal Endothelial Cells.Journal of clinical medicine · 2022Article
- PKA and Epac1 Reduce Nek7 to Block the NLRP3 Inflammasome Proteins in the Retinal Vasculature.Investigative ophthalmology & visual science · 2022Article
- TNFAIP3 is anti-inflammatory in the retinal vasculature.Molecular vision · 2022Article
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- Forskolin eye drops improve retinal damage from ischemia/reperfusion.Molecular vision · 2021Article
- Epac1 Requires AMPK Phosphorylation to Regulate HMGB1 in the Retinal Vasculature.Investigative ophthalmology & visual science · 2020Article
- Review: Role of cAMP signaling in diabetic retinopathy.Molecular vision · 2020Review
- Epac1 and Glycyrrhizin Both Inhibit HMGB1 Levels to Reduce Diabetes-Induced Neuronal and Vascular Damage in the Mouse Retina.Journal of clinical medicine · 2019Article
- Epac1 inhibits PKR to reduce NLRP3 inflammasome proteins in retinal endothelial cells.Journal of inflammation research · 2019Article
- PKA regulates HMGB1 through activation of IGFBP-3 and SIRT1 in human retinal endothelial cells cultured in high glucose.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2018Article
- HMGB1 inhibits insulin signalling through TLR4 and RAGE in human retinal endothelial cells.Growth factors (Chur, Switzerland) · 2018Article
- Epac1 protects the retina against ischemia/reperfusion-induced neuronal and vascular damage.PloS one · 2018Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
purposeIncreased inflammatory mediator levels are reported in diabetic retinopathy. We previously reported that β-adrenergic receptor agonists reduced inflammatory mediators in the diabetic retina; however, these agents cannot be given systemically. Here, we investigated whether Epac1 is key to the protective effects of β-adrenergic receptor agonists.
methodsWe cultured primary human retinal endothelial cells (RECs) in normal (5 mM) or high (25 mM) glucose and treated them with an Epac1-specific agonist. Additionally, we generated Epac1 conditional vascular endothelial cell knockout mice by breeding Epac1 floxed mice with Cdh5 Cre mice to investigate the role of Epac1 in the retinal levels of tumor necrosis factor alpha (TNF-α), interleukin-1β (IL-1β), nuclear factor kappa beta (NFκB), and inhibitor of kappa beta (IκB). Confocal microscopy was performed to localize Epac1 in the mouse retina.
resultsData showed that high glucose increased the TNF-α and IL-1β levels in the RECs, which were reduced cells treated with the Epac1 agonist. The loss of Epac1 in the retinas of the conditional knockout mice resulted in statistically significantly increased levels of TNF-α and IL-1β, as well as NFκB.
conclusionsThese data indicate that Epac1 may be protective to the retina through inhibition of key inflammatory mediators.
Indexed as
Identifiers
28210097PMC5287445W2584347113What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.