Evidence map›Paper›PMID 28209589›Full record

ArticleGenetics2017

Second-Generation

Ben Sutcliffe, Julian Ng, Thomas O Auer, Mathias Pasche, Richard Benton, Gregory S X E Jefferis, Sebastian Cachero

Abstract read
In one paragraph

Article in Genetics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed.

  1. Article
  2. Disruption of a selective vesicle pool upon retrograde amnesia dissociates memory at presynaptic terminals.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  3. Review
  4. Twists to Classical Conditioning of AdultCold Spring Harbor protocols · 2025
    Article
  5. Article
  6. Article
  7. Experimental Approaches to Visualize Effector Protein Translocation During Host-Pathogen Interactions.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025
    Review
  8. Article
  9. The sparse driver system forbioRxiv : the preprint server for biology · 2024
    Article
  10. Rewarding Capacity of Optogenetically Activating a Giant GABAergic Central-Brain Interneuron in LarvalThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2023
    Article
  11. Article
  12. Cell-Surface Targeting of Fluorophores inACS chemical neuroscience · 2023
    Article
  13. IntacteLife · 2022
    Article
  14. Article
  15. Article
  16. Voltage Imaging inFrontiers in neuroscience · 2021
    Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Ben SutcliffeDivision of Neurobiology, MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK.ORCID 0000-0003-3264-1649
Julian NgDivision of Neurobiology, MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK.ORCID 0000-0002-1908-4414
Thomas O AuerCenter for Integrative Genomics, Faculty of Biology and Medicine, University of Lausanne, CH-1015, Switzerland.
Mathias PascheDivision of Cell Biology, MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK.ORCID 0000-0002-9347-383X
Richard BentonCenter for Integrative Genomics, Faculty of Biology and Medicine, University of Lausanne, CH-1015, Switzerland.ORCID 0000-0003-4305-8301
Gregory S X E JefferisDivision of Neurobiology, MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK jefferis@mrc-lmb.cam.ac.uk scachero@mrc-lmb.cam.ac.uk.ORCID 0000-0002-0587-9355
Sebastian CacheroDivision of Neurobiology, MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK jefferis@mrc-lmb.cam.ac.uk scachero@mrc-lmb.cam.ac.uk.ORCID 0000-0002-6828-1211

Funding

Resource Component: Acquisition, maintenance and distribution of Drosophila stocksP40OD018537 · OD · TRUSTEES OF INDIANA UNIVERSITY · PI Annette L. Parks · 2014 to 2026
$13.5M
European Research Council 211089European Research Council 615094Medical Research Council MC_U105178788Medical Research Council MC_U105188491NIH HHS P40 OD018537
6 · The paper itself

Abstract

Labeling and visualizing cells and subcellular structures within thick tissues, whole organs, and even intact animals is key to studying biological processes. This is particularly true for studies of neural circuits where neurons form submicron synapses but have arbors that may span millimeters in length. Traditionally, labeling is achieved by immunofluorescence; however, diffusion of antibody molecules (>100 kDa) is slow and often results in uneven labeling with very poor penetration into the center of thick specimens; these limitations can be partially addressed by extending staining protocols to over a week (

Indexed as

Alkyl and Aryl TransferasesAnimalsBrainDrosophilaDrosophila ProteinsFluorescent DyesMicroscopy, FluorescenceOptical ImagingRecombinant ProteinsSensitivity and SpecificityStaining and LabelingAlkyl and Aryl TransferasesDNA alkyltransferaseDrosophila ProteinsFluorescent DyesRecombinant Proteinschemical labelingchemical tagsfluorescence microscopyimmunohistochemistryneural circuitsprotein labeling

Identifiers

PMID28209589
PMCPMC5378102

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.