ArticleJournal of clinical and diagnostic research : JCDR2016
Polymerase Chain Reaction: An Important Tool for Early Diagnosis of Leptospirosis Cases.
Article in Journal of clinical and diagnostic research : JCDR, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 3 of them syntheses that pooled it.
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Who cites it
25 citing papers in PubMed, 3 syntheses or guidelines pooled it.
- Diagnosis of human leptospirosis: systematic review and meta-analysis of the diagnostic accuracy of the Leptospira microscopic agglutination test, PCR targeting Lfb1, and IgM ELISA to Leptospira fainei serovar Hurstbridge.BMC infectious diseases · 2024Pooled it
- Diagnostic accuracy of genetic markers and nucleic acid techniques for the detection of Leptospira in clinical samples: A meta-analysis.PLoS neglected tropical diseases · 2020Pooled it
- Nucleic acid and antigen detection tests for leptospirosis.The Cochrane database of systematic reviews · 2019Pooled it
- Clinico-Epidemiological Profile of Leptospirosis Among Patients Attending a Tertiary Care Center in Rajasthan.Cureus · 2026Article
- Retrospective Molecular Detection and Characterization of PathogenicTropical medicine and infectious disease · 2026Article
- Leptospirosis and Respiratory Syncytial Virus (RSV) Co-infection: Diagnostic and Management Challenges.Cureus · 2025Article
- Diagnostic Advances in Leptospirosis: A Comparative Analysis of Paraclinical Tests with a Focus on PCR.Microorganisms · 2025Review
- Leptospirosis in humans and animals in Malaysia: A review from 1976 to 2023.Veterinary world · 2025Review
- Evaluation of Serological Tests for Different Disease Stages of Leptospirosis Infection in Humans.Tropical medicine and infectious disease · 2024Article
- Clinical presentation of human leptospirosis in febrile patients: Urabá, Colombia.PLoS neglected tropical diseases · 2024Observational
- Leptospirosis: a clinical and diagnostic challenge.BMJ case reports · 2024Article
- Infection and re-infection ofVeterinary world · 2024Article
- Relevance of Polymerase Chain Reaction in Early Diagnosis of Leptospirosis.Indian journal of critical care medicine : peer-reviewed, official publication of Indian Society of Critical Care Medicine · 2024Article
- Molecular Characterization of Gene Encoding Outer Membrane ProteinJournal of tropical medicine · 2024Article
- Molecular Diagnosis as an Alternative for Public Health Surveillance of Leptospirosis in Colombia.Microorganisms · 2023Review
- Effect of Demographics and Time to Sample Processing on the qPCR Detection of PathogenicTropical medicine and infectious disease · 2023Article
- Molecular and Serological Identification of PathogenicTransboundary and emerging diseases · 2023Article
- Comparison of anti-Leptospira antibodies by microscopic agglutination test in ruminants and equines of Urmia, Iran.Veterinary research forum : an international quarterly journal · 2023Article
- Exposure of Free-Ranging Wild Animals to ZoonoticAnimals : an open access journal from MDPI · 2021Article
- Leptospirosis as a cause of fever associated with jaundice in the Democratic Republic of the Congo.PLoS neglected tropical diseases · 2021Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionVarious diagnostic methods like Microscopic Agglutination Test (MAT), IgM ELISA, Isolation of
aimThe aim of the study was to detect the leptospiral DNA by PCR method and to compare the results of PCR with other available diagnostic methods used for diagnosis of suspected leptospirosis cases in acute phase of illness. MATERIALS AND
methodsA total of 207 blood samples were obtained from suspected patients of leptospirosis admitted in New Civil Hospital, a tertiary care hospital in South Gujarat, during the period of July 2008 to November 2008. These blood samples were subjected to Rapid leptocheck, IgM ELISA, MAT test to detect (IgG or IgM) antibody level,
resultsIn early phase of the disease, Rapid leptocheck test gave 44% detection, but along with PCR seropositivity reached upto 71%. Detection rate by IgM ELISA was 59% which increased to 80% with PCR. By MAT seropositivity was 57% but combined seropositivity of MAT with PCR was 78%. Sensitivity and specificity of PCR as compared to MAT (Gold standard) was 52% and 79% respectively.
conclusionIn present study, PCR picked up to 50% of cases which were negative by other serological tests so these finding suggest that PCR should be used routinely in acute phase of disease.
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