ArticleMolecules (Basel, Switzerland)2017
Novel Magnetic Cross-Linked Cellulase Aggregates with a Potential Application in Lignocellulosic Biomass Bioconversion.
Article in Molecules (Basel, Switzerland), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Recycling the recyclers: strategies for the immobilisation of a PET-degrading cutinase.Bioprocess and biosystems engineering · 2025Article
- Nano-based biofuel production from low-cost lignocellulose biomass: environmental sustainability and economic approach.Bioprocess and biosystems engineering · 2024Review
- Review
- Tyrosinase Magnetic Cross-Linked Enzyme Aggregates: Biocatalytic Study in Deep Eutectic Solvent Aqueous Solutions.Biomolecules · 2023Article
- (Magnetic) Cross-Linked Enzyme Aggregates of Cellulase fromMolecules (Basel, Switzerland) · 2023Article
- Cellulase Immobilization on Nanostructured Supports for Biomass Waste Processing.Nanomaterials (Basel, Switzerland) · 2022Review
- Preparation and Characterization of Magnetic Metal-Organic Frameworks Functionalized by Ionic Liquid as Supports for Immobilization of Pancreatic Lipase.Molecules (Basel, Switzerland) · 2022Article
- Biotechnological advances in biomass pretreatment for bio-renewable production through nanotechnological intervention.Biomass conversion and biorefinery · 2022Review
- Magnetic COFs as satisfied support for lipase immobilization and recovery to effectively achieve the production of biodiesel by great maintenance of enzyme activity.Biotechnology for biofuels · 2021Article
- Cellulases: From Bioactivity to a Variety of Industrial Applications.Biomimetics (Basel, Switzerland) · 2021Review
- Innovations in CAZyme gene diversity and its modification for biorefinery applications.Biotechnology reports (Amsterdam, Netherlands) · 2020Review
- Crosslinked Enzyme Aggregates (CLEAs) of Laccases fromMolecules (Basel, Switzerland) · 2020Article
- Affinity Immobilization of a Bacterial Prolidase onto Metal-Ion-Chelated Magnetic Nanoparticles for the Hydrolysis of Organophosphorus Compounds.International journal of molecular sciences · 2019Article
- Review
- CALB Immobilized onto Magnetic Nanoparticles for Efficient Kinetic Resolution of Racemic Secondary Alcohols: Long-Term Stability and Reusability.Molecules (Basel, Switzerland) · 2019Article
- Improved Performance of Magnetic Cross-Linked Lipase Aggregates by Interfacial Activation: A Robust and Magnetically Recyclable Biocatalyst for Transesterification of Jatropha Oil.Molecules (Basel, Switzerland) · 2017Article
- Biosynthesis of S-Adenosylmethionine by Magnetically Immobilized Escherichia coli Cells Highly Expressing a Methionine Adenosyltransferase Variant.Molecules (Basel, Switzerland) · 2017Article
- Special Issue: Enzyme Immobilization 2016.Molecules (Basel, Switzerland) · 2017Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The utilization of renewable biomass resources to produce high-value chemicals by enzymatic processes is beneficial for alternative energy production, due to the accelerating depletion of fossil fuels. As immobilization techniques can improve enzyme stability and reusability, a novel magnetic cross-linked cellulase aggregate has been developed and applied for biomass bioconversion. The crosslinked aggregates could purify and immobilize enzymes in a single operation, and could then be combined with magnetic nanoparticles (MNPs), which provides easy separation of the materials. The immobilized cellulase showed a better activity at a wider temperature range and pH values than that of the free cellulase. After six cycles of consecutive reuse, the immobilized cellulase performed successful magnetic separation and retained 74% of its initial activity when carboxylmethyl cellulose (CMC) was used as the model substrate. Furthermore, the structure and morphology of the immobilized cellulase were studied by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Moreover, the immobilized cellulase was shown to hydrolyze bamboo biomass with a yield of 21%, and was re-used in biomass conversion up to four cycles with 38% activity retention, which indicated that the immobilized enzyme has good potential for biomass applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.