ArticleMicroorganisms2017
Fluorescence Lectin Bar-Coding of Glycoconjugates in the Extracellular Matrix of Biofilm and Bioaggregate Forming Microorganisms.
Article in Microorganisms, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 56 citations in OpenAlex.
- Biofilm Analysis by Confocal Microscopy-Basics and Practical Aspects.Microscopy research and technique · 2026Review
- Sugar supplementation enhances biofilm formation and extracellular polysaccharides production inFrontiers in microbiology · 2026Article
- Evolution of genotypic and phenotypic diversity in multispecies biofilms.NPJ biofilms and microbiomes · 2025Article
- Article
- Molecular choreography of sludge extracellular polymeric substances-From biomolecule identification to energetics and assembly dynamics.PNAS nexus · 2025Article
- Alterations of Glycan Composition in Aerobic Granular Sludge during the Adaptation to Seawater Conditions.ACS ES&T water · 2024Article
- Progress in bioleaching: fundamentals and mechanisms of microbial metal sulfide oxidation - part A.Applied microbiology and biotechnology · 2022Review
- Matrix glycoconjugate characterization in multispecies biofilms and bioaggregates from the environment by means of fluorescently-labeled lectins.Frontiers in microbiology · 2022Article
- Production of nonulosonic acids in the extracellular polymeric substances of "Candidatus Accumulibacter phosphatis".Applied microbiology and biotechnology · 2021Article
- Decorating the Anammox House: Sialic Acids and Sulfated Glycosaminoglycans in the Extracellular Polymeric Substances of Anammox Granular Sludge.Environmental science & technology · 2020Article
- Glycomics Microarrays Reveal Differential In Situ Presentation of the Biofilm Polysaccharide Poly-International journal of molecular sciences · 2020Article
- Soil aggregates stability and storage of soil organic carbon respond to cropping systems on Black Soils of Northeast China.Scientific reports · 2020Article
- Insight Into Interactions of Thermoacidophilic Archaea With Elemental Sulfur: Biofilm Dynamics and EPS Analysis.Frontiers in microbiology · 2019Article
- Application of cationic polymer micelles for the dispersal of bacterial biofilms.Engineering in life sciences · 2018Article
- Identification of Glycoproteins Isolated from Extracellular Polymeric Substances of Full-Scale Anammox Granular Sludge.Environmental science & technology · 2018Article
- EPS Glycoconjugate Profiles Shift as Adaptive Response in Anaerobic Microbial Granulation at High Salinity.Frontiers in microbiology · 2018Article
- Osteopontin adsorption to Gram-positive cells reduces adhesion forces and attachment to surfaces under flow.Journal of oral microbiology · 2017Article
- Visualizing the dental biofilm matrix by means of fluorescence lectin-binding analysis.Journal of oral microbiology · 2017Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microbial biofilm systems are defined as interface-associated microorganisms embedded into a self-produced matrix. The extracellular matrix represents a continuous challenge in terms of characterization and analysis. The tools applied in more detailed studies comprise extraction/chemical analysis, molecular characterization, and visualisation using various techniques. Imaging by laser microscopy became a standard tool for biofilm analysis, and, in combination with fluorescently labelled lectins, the glycoconjugates of the matrix can be assessed. By employing this approach a wide range of pure culture biofilms from different habitats were examined using the commercially available lectins. From the results, a binary barcode pattern of lectin binding can be generated. Furthermore, the results can be fine-tuned and transferred into a heat map according to signal intensity. The lectin barcode approach is suggested as a useful tool for investigating the biofilm matrix characteristics and dynamics at various levels, e.g. bacterial cell surfaces, adhesive footprints, individual microcolonies, and the gross biofilm or bio-aggregate. Hence fluorescence lectin bar-coding (FLBC) serves as a basis for a subsequent tailor-made fluorescence lectin-binding analysis (FLBA) of a particular biofilm. So far, the lectin approach represents the only tool for in situ characterization of the glycoconjugate makeup in biofilm systems. Furthermore, lectin staining lends itself to other fluorescence techniques in order to correlate it with cellular biofilm constituents in general and glycoconjugate producers in particular.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.