Evidence map›Paper›PMID 28207864›Full record

ArticlePloS one2017

Characterisation and genome sequence of the lytic Acinetobacter baumannii bacteriophage vB_AbaS_Loki.

Dann Turner, Matthew E Wand, Yves Briers, Rob Lavigne, J Mark Sutton, Darren M Reynolds

Open access · goldAbstract read
In one paragraph

Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed, 1 pooled it
3.1field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed, 1 synthesis or guideline pooled it, 41 citations in OpenAlex.

  1. Pooled it
  2. Isolation and characterization of two phages infectingThe Journal of general virology · 2026
    Article
  3. Article
  4. Review
  5. Article
  6. Article
  7. Article
  8. Review
  9. Article
  10. Lytic Capsule-SpecificViruses · 2024
    Article
  11. Functional domains ofFrontiers in microbiology · 2024
    Article
  12. Potential Usefulness of Bacteriophages for the Treatment of Multidrug-ResistantThe Malaysian journal of medical sciences : MJMS · 2023
    Review
  13. Article
  14. Review
  15. Article
  16. Chitosan-Based Microparticle EncapsulatedTurkish journal of pharmaceutical sciences · 2022
    Article
  17. Article
  18. Review
  19. Lysin LysMK34 ofApplied and environmental microbiology · 2020
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 2 countries.

Dann TurnerCentre for Research in Biosciences, Department of Applied Sciences, Faculty of Health and Applied Sciences, University of the West of England, Coldharbour Lane, Bristol, United Kingdom.ORCID http://orcid.org/0000-0002-0249-4513
Matthew E WandNational Infections Service, Public Health England, Porton Down, Salisbury, Wiltshire, United Kingdom.
Yves BriersLaboratory of Applied Biotechnology, Department of Applied Biosciences, Ghent University, Ghent, Belgium.
Rob LavigneLaboratory of Gene Technology, Biosystems Department, KU Leuven, Heverlee, Belgium.
J Mark SuttonNational Infections Service, Public Health England, Porton Down, Salisbury, Wiltshire, United Kingdom.
Darren M ReynoldsCentre for Research in Biosciences, Department of Applied Sciences, Faculty of Health and Applied Sciences, University of the West of England, Coldharbour Lane, Bristol, United Kingdom.
Public Health England · GBUniversity of the West of England · GBGhent University · BEKU Leuven · BE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Acinetobacter baumannii has emerged as an important nosocomial pathogen in healthcare and community settings. While over 100 of Acinetobacter phages have been described in the literature, relatively few have been sequenced. This work describes the characterisation and genome annotation of a new lytic Acinetobacter siphovirus, vB_AbaS_Loki, isolated from activated sewage sludge. Sequencing revealed that Loki encapsulates a 41,308 bp genome, encoding 51 predicted open reading frames. Loki is most closely related to Acinetobacter phage IME_AB3 and more distantly related to Burkholderia phage KL1, Paracoccus phage vB_PmaS_IMEP1 and Pseudomonas phages vB_Pae_Kakheti25, vB_PaeS_SCH_Ab26 and PA73. Loki is characterised by a narrow host range, among the 40 Acinetobacter isolates tested, productive infection was only observed for the propagating host, A. baumannii ATCC 17978. Plaque formation was found to be dependent upon the presence of Ca2+ ions and adsorption to host cells was abolished upon incubation with a mutant of ATCC 17978 encoding a premature stop codon in lpxA. The complete genome sequence of vB_AbaS_Loki was deposited in the European Nucleotide Archive (ENA) under the accession number LN890663.

Indexed as

Genome, ViralAcinetobacter baumanniiAcinetobacter InfectionsBacteriophagesDNA, ViralHumansPhylogenySequence Analysis, DNAViral Plaque AssayViral ProteinsVirus AttachmentDNA, ViralViral Proteins

Identifiers

PMID28207864
PMCPMC5313236
OpenAlexW2588479553

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.