ArticleScientific reports2017
System-wide detection of protein-small molecule complexes suggests extensive metabolite regulation in plants.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 45 citations in OpenAlex.
- MsMTA regulates growth and metabolism in alfalfa (Horticulture research · 2026Article
- Discovery of regulatory complexes in Arabidopsis via protein-metabolite mapping during Pseudomonas infection.Plant physiology · 2026Article
- Not to be overlooked: dipeptides and their role in plant stress resilience.Journal of experimental botany · 2025Review
- The recruitment of the A-type cyclin TAM to stress granules is crucial for meiotic fidelity under heat.Science advances · 2025Article
- Mapping protein-metabolite interactions iniScience · 2025Article
- Interrogating stonefish venom: small molecules present in envenomation caused by Synanceia spp.FEBS open bio · 2025Article
- Insights into the metabolism, signaling, and physiological effects of 2',3'-cyclic nucleotide monophosphates in bacteria.Critical reviews in biochemistry and molecular biology · 2023Review
- Review
- Rewiring of the protein-protein-metabolite interactome during the diauxic shift in yeast.Cellular and molecular life sciences : CMLS · 2022Article
- Cellular Effects of 2',3'-Cyclic Nucleotide Monophosphates in Gram-Negative Bacteria.Journal of bacteriology · 2022Article
- Regulatory Modules of Metabolites and Protein Phosphorylation in Arabidopsis Genotypes With Altered Sucrose Allocation.Frontiers in plant science · 2022Article
- Tyr-Asp inhibition of glyceraldehyde 3-phosphate dehydrogenase affects plant redox metabolism.The EMBO journal · 2021Article
- Mapping the plant proteome: tools for surveying coordinating pathways.Emerging topics in life sciences · 2021Review
- Global mapping of protein-metabolite interactions in Saccharomyces cerevisiae reveals that Ser-Leu dipeptide regulates phosphoglycerate kinase activity.Communications biology · 2021Article
- Proteogenic Dipeptides Are Characterized by Diel Fluctuations and Target of Rapamycin Complex-Signaling Dependency in the Model PlantFrontiers in plant science · 2021Article
- PROMISed: A novel web-based tool to facilitate analysis and visualization of the molecular interaction networks from co-fractionation mass spectrometry (CF-MS) experiments.Computational and structural biotechnology journal · 2021Article
- Emerging strategies for the identification of protein-metabolite interactions.Journal of experimental botany · 2019Article
- PROMIS, global analysis of PROtein-metabolite interactions using size separation inThe Journal of biological chemistry · 2018Article
- 2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes.Journal of visualized experiments : JoVE · 2018Article
- Interaction of 2',3'-cAMP with Rbp47b Plays a Role in Stress Granule Formation.Plant physiology · 2018Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein small molecule interactions are at the core of cell regulation controlling metabolism and development. We reasoned that due to the lack of system wide approaches only a minority of those regulatory molecules are known. In order to see whether or not this assumption is true we developed an effective approach for the identification of small molecules having potential regulatory role that obviates the need of protein or small molecule baits. At the core of this approach is a simple biochemical co-fractionation taking advantage of size differences between proteins and small molecules. Metabolomics based analysis of small molecules co-fractionating with proteins identified a multitude of small molecules in Arabidopsis suggesting the existence of numerous, small molecules/metabolites bound to proteins representing potential regulatory molecules. The approach presented here uses Arabidopsis cell cultures, but is generic and hence applicable to all biological systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.