Evidence map›Paper›PMID 28205268›Full record

ArticleCell proliferation2017

The effect of delta-like 1 homologue on the proliferation and odontoblastic differentiation in human dental pulp stem cells.

Shengcai Qi, Yanhong Yan, Yue Wen, Jialiang Li, Jing Wang, Fubo Chen, Xiaoshan Tang, Guangwei Shang, Yuanzhi Xu, Raorao Wang

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell proliferation, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.1field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 9 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Shengcai QiDepartment of Stomatology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.
Yanhong YanDepartment of Pediatric Dentistry, School & Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai, China.
Yue WenInstitute of Stomatology, Hospital of Stomatology, Nanjing Medical University, Nanjing, China.
Jialiang LiInstitute of Stomatology, Hospital of Stomatology, Nanjing Medical University, Nanjing, China.
Jing WangDepartment of Stomatology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.
Fubo ChenDepartment of Stomatology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.
Xiaoshan TangDepartment of Stomatology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.
Guangwei ShangDepartment of Stomatology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.
Yuanzhi XuDepartment of Stomatology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.
Raorao WangDepartment of Stomatology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.ORCID http://orcid.org/0000-0002-0224-3255
Tongji University · CNStomatology Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionThis study aimed to investigate the functions of delta-like homologue 1 (DLK1) in the proliferation and differentiation of human dental pulp stem cells (hDPSCs).

methodsImmunohistochemical analysis was used to determine the expression of alkaline phosphatase (ALP), dentin sialophosphoprotein (DSPP), DLK1, NOTCH1 and p-ERK1/2 in the mouse first maxillary molar. Recombinant lentivirus was constructed to overexpress DLK1 stably in hDPSCs. The cell viability and proliferation of hDPSCs were examined by CCK8 and EdU incorporation assay respectively. The odontoblastic differentiation of hDPSCs was determined by detection of ALPase activity assay, ALP and alizarin red staining and the expression of mineralization-related genes including ALP, DSPP and dental matrix protein. The mRNA and protein levels of DLK1 and p-ERK1/2 protein expression were detected. ERK inhibitor was used to test the differentiation effect of DLK1 on hDPSCs.

resultsDelta-like homologue 1 was highly expressed on the odontoblasts and dental pulp cells on the first maxillary molar; the expression of p-ERK1/2 is similar with the DLK1 in the same process. The expression level of DLK1 increased significantly after the odontoblastic induction of hDPSCs. DLK1 overexpression increased the proliferation ability of hDPSCs and inhibited odontoblastic differentiation of hDPSCs. The protein level of p-ERK1/2 significantly increased in hDPSCs/dlk1-oe group. ERK signalling pathway inhibitor reversed the odontoblastic differentiation effects of DLK1 on hDPSCs.

conclusionsThe proliferation of hDPSCs was promoted after DLK1 overexpression. DLK1 inhibited the odontoblastic differentiation of hDPSCs, which maybe through ERK signalling pathway.

Indexed as

Cell DifferentiationCell ProliferationAnimalsCalcium-Binding ProteinsCells, CulturedDental PulpHumansIntercellular Signaling Peptides and ProteinsMembrane ProteinsMiceMice, Inbred C57BLOdontoblastsStem CellsCalcium-Binding ProteinsDLK1 protein, humanIntercellular Signaling Peptides and ProteinsMembrane Proteins

Identifiers

PMID28205268
PMCPMC6529117
OpenAlexW2588550554

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.