Evidence map›Paper›PMID 28205170›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2017

Transport Assays for Sterol-Binding Proteins: Stopped-Flow Fluorescence Methods for Investigating Intracellular Cholesterol Transport Mechanisms of NPC2 Protein.

Leslie A McCauliff, Judith Storch

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Leslie A McCauliffDepartment of Nutritional Sciences and Rutgers Center for Lipid Research, Rutgers University New Brunswick, 65 Dudley Road, New Brunswick, NJ, 08901, USA.
Judith StorchDepartment of Nutritional Sciences and Rutgers Center for Lipid Research, Rutgers University New Brunswick, 65 Dudley Road, New Brunswick, NJ, 08901, USA. storch@aesop.rutgers.edu.

Funding

Supplement to DK-38389 Lipid Transport in the IntestineR01DK038389 · NIDDK · RUTGERS THE ST UNIV OF NJ NEW BRUNSWICK · PI SAMPATH, HARINI, STORCH, JUDITH · 1992 to 2025
$8.6M
Mechanisms of Cholesterol Transport by NPC ProteinsR01GM115866 · NIGMS · RUTGERS, THE STATE UNIV OF N.J. · PI STORCH, JUDITH · 2016 to 2019
$1.1M
NIDDK NIH HHS R01 DK038389NIGMS NIH HHS R01 GM115866
6 · The paper itself

Abstract

In this chapter we describe the use of stopped flow fluorescence spectroscopy to analyze the kinetic mechanisms of protein mediated cholesterol transfer to, from, and between model membranes. These assays allow for the detection of protein-membrane interactions that may occur during cholesterol transfer by simply modifying donor or acceptor concentrations, membrane composition, or buffer properties, and analyzing resultant transfer rates.

Indexed as

Membranes, ArtificialBiological Transport, ActiveCarrier ProteinsCholesterolGlycoproteinsHumansKineticsSpectrometry, FluorescenceVesicular Transport ProteinsCarrier ProteinsCholesterolGlycoproteinsMembranes, ArtificialNPC2 protein, humanVesicular Transport ProteinsCholesterolCholesterol traffickingCollisional transferDiffusional transferFluorescenceFRETModel membranesSmall unilamellar vesiclesStopped flow

Identifiers

PMID28205170
PMCPMC6311532

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.