ArticleInternational journal of molecular medicine2017
Neutrophil extracellular traps induce IL-1β production by macrophages in combination with lipopolysaccharide.
Article in International journal of molecular medicine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
47 citing papers in PubMed, 1 synthesis or guideline pooled it, 72 citations in OpenAlex.
- Severe COVID-19: what have we learned with the immunopathogenesis?Advances in rheumatology (London, England) · 2020Pooled it
- The Role of Myeloperoxidase in Osteoarthritis and Its Potential Modulation by Resveratrol: An Integrated Study.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Article
- Neutrophil Extracellular Traps Promote the Formation of Canine Dental Calculus.Veterinary sciences · 2026Article
- [The Oral Microbiome: Maintenance of Homeostasis, Disease Associations, and Mechanisms of Pathogenesis].Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2026Review
- Neutrophil extracellular traps in gynecological disease: pathogenic mechanisms and therapeutic opportunities.Frontiers in medicine · 2026Review
- NETs accelerate aortic valve calcification by promoting M1 macrophage polarization through the TLR9 signaling pathway.Molecular and cellular biochemistry · 2025Article
- Neutrophil extracellular traps drive osteoporosis via NCF2-dependent signaling: integrated transcriptomics with mechanistic validation.Redox report : communications in free radical research · 2025Article
- Neutrophils and NETs in Pathophysiology and Treatment of Inflammatory Bowel Disease.International journal of molecular sciences · 2025Review
- Hypothermia protects against ventilator-induced lung injury by limiting IL-1β release and NETs formation.eLife · 2025Article
- Hypothermia protects against ventilator-induced lung injury by limiting IL-1β release and NETs formation.bioRxiv : the preprint server for biology · 2025Article
- The emerging role of neutrophil extracellular traps in autoimmune and autoinflammatory diseases.MedComm · 2025Review
- FeMOFs/CO loading reduces NETosis and macrophage inflammatory response in PLA based cardiovascular stent materials.Regenerative biomaterials · 2025Article
- Crosstalk between neutrophil extracellular traps and gut microbiota in ulcerative colitis: traditional Chinese medicine strategies.Frontiers in cellular and infection microbiology · 2025Review
- Return to Intended Oncological Therapy: State of the Art and Perspectives.Current oncology reports · 2024Review
- Broader Perspective on Atherosclerosis-Selected Risk Factors, Biomarkers, and Therapeutic Approach.International journal of molecular sciences · 2024Review
- Cayratia albifolia C.L.Li exerts anti-rheumatoid arthritis effect by inhibiting macrophage activation and neutrophil extracellular traps (NETs).Chinese medicine · 2024Article
- Bacteriophages isolated from mouse feces attenuates pneumonia mice caused by Pseudomonas aeruginosa.PloS one · 2024Article
- The emerging role of neutrophil extracellular traps in ulcerative colitis.Frontiers in immunology · 2024Review
- Lipoprotein(a), Interleukin-6 inhibitors, and atherosclerotic cardiovascular disease: Is there an association?Atherosclerosis plus · 2023Review
- Extracellular Mechanisms of Neutrophils in Immune Cell Crosstalk.Immune network · 2023Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Upon exposure to invading microorganisms, neutrophils undergo NETosis, a recently identified type of programmed cell death, and release neutrophil extracellular traps (NETs). NETs are described as an antimicrobial mechanism, based on the fact that NETs can trap microorganisms and exhibit bactericidal activity through the action of NET‑associated components. In contrast, the components of NETs have been recognized as damage‑associated molecular pattern molecules (DAMPs), which trigger inflammatory signals to induce cell death, inflammation and organ failure. In the present study, to clarify the effect of NETs on cytokine production by macrophages, mouse macrophage‑like J774 cells were treated with NETs in combination with lipopolysaccharide (LPS) as a constituent of pathogen‑associated molecular patterns. The results revealed that NETs significantly induced the production of interleukin (IL)‑1β by J774 cells in the presence of LPS. Notably, the NET/LPS‑induced IL‑1β production was inhibited by both caspase‑1 and caspase‑8 inhibitors. Furthermore, nucleases and serine protease inhibitors but not anti‑histone antibodies significantly inhibited the NET/LPS‑induced IL‑1β production. Moreover, we confirmed that caspase‑1 and caspase‑8 were activated by NETs/LPS, and the combination of LPS, DNA and neutrophil elastase induced IL‑1β production in reconstitution experiments. These observations indicate that NETs induce the production of IL‑1β by J774 macrophages in combination with LPS via the caspase‑1 and caspase‑8 pathways, and NET‑associated DNA and serine proteases are involved in NET/LPS‑induced IL‑1β production as essential components.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.