ArticleAnnals of surgical treatment and research2017
Three-dimensional (3D) printing of mouse primary hepatocytes to generate 3D hepatic structure.
Article in Annals of surgical treatment and research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed.
- Decoding triple negative breast cancer bone metastasis: from 3D bioprinted models to clinical translation.Journal of nanobiotechnology · 2026Review
- Liver organoids: Current advances and future applications for hepatology.Clinical and molecular hepatology · 2025Review
- 3D bioprinting of liver models: A systematic scoping review of methods, bioinks, and reporting quality.Materials today. Bio · 2024Article
- Evolution of Hybrid Hydrogels: Next-Generation Biomaterials for Drug Delivery and Tissue Engineering.Gels (Basel, Switzerland) · 2024Review
- Three Dimensional Bioprinting for Hepatic Tissue Engineering: From In Vitro Models to Clinical Applications.Tissue engineering and regenerative medicine · 2024Review
- Progress in bioprinting technology for tissue regeneration.Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs · 2023Review
- Microfluidic Liver-on-a-Chip for Preclinical Drug Discovery.Pharmaceutics · 2023Review
- Out of Box Thinking to Tangible Science: A Benchmark History of 3D Bio-Printing in Regenerative Medicine and Tissues Engineering.Life (Basel, Switzerland) · 2023Review
- In Vitro Models for the Study of Liver Biology and Diseases: Advances and Limitations.Cellular and molecular gastroenterology and hepatology · 2023Review
- [Progress in research and development of soft tissue three-dimensional bioprinting and its supporting equipment].Zhonghua shao shang yu chuang mian xiu fu za zhi · 2022Article
- Printability and Cell Viability in Extrusion-Based Bioprinting from Experimental, Computational, and Machine Learning Views.Journal of functional biomaterials · 2022Review
- Review
- The Performance of HepG2 and HepaRG Systems through the Glass of Acetaminophen-Induced Toxicity.Life (Basel, Switzerland) · 2021Article
- Emerging liver organoid platforms and technologies.Cell regeneration (London, England) · 2021Review
- Advances in 3D Printing for Tissue Engineering.Materials (Basel, Switzerland) · 2021Review
- The Synergy between Organ-on-a-Chip and Artificial Intelligence for the Study of NAFLD: From Basic Science to Clinical Research.Biomedicines · 2021Review
- Solid Organ Bioprinting: Strategies to Achieve Organ Function.Chemical reviews · 2020Review
- Emulating Human Tissues and Organs: A Bioprinting Perspective Toward Personalized Medicine.Chemical reviews · 2020Review
- Current progress in hepatic tissue regeneration by tissue engineering.Journal of translational medicine · 2019Review
- Bioprinting for Liver Transplantation.Bioengineering (Basel, Switzerland) · 2019Review
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThe major problem in producing artificial livers is that primary hepatocytes cannot be cultured for many days. Recently, 3-dimensional (3D) printing technology draws attention and this technology regarded as a useful tool for current cell biology. By using the 3D bio-printing, these problems can be resolved.
methodsTo generate 3D bio-printed structures (25 mm × 25 mm), cells-alginate constructs were fabricated by 3D bio-printing system. Mouse primary hepatocytes were isolated from the livers of 6-8 weeks old mice by a 2-step collagenase method. Samples of 4 × 10
resultsIsolated primary hepatocytes were printed with alginate. The 3D printed hepatocytes remained alive for 14 days. Gene expression levels of
conclusionOur research indicates that 3D bio-printing technique can be used for long-term culture of primary hepatocytes. It can therefore be used for drug screening and as a potential method of producing artificial livers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.