ArticleJournal of neurophysiology2017
AAV-mediated delivery of optogenetic constructs to the macaque brain triggers humoral immune responses.
Article in Journal of neurophysiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 36 citations in OpenAlex.
- Optotagging in primate cortex uncovers excitatory and inhibitory neuron specializations in form vision.Nature communications · 2026Article
- Optogenetics and Targeted Gene Therapy for Retinal Diseases: Unravelling the Fundamentals, Applications, and Future Perspectives.Journal of clinical medicine · 2024Review
- Direct Comparison of Epifluorescence and Immunostaining for Assessing Viral Mediated Gene Expression in the Primate Brain.Human gene therapy · 2023Article
- Microscale multicircuit brain stimulation: Achieving real-time brain state control for novel applications.Current research in neurobiology · 2023Review
- A comparison of the impact on neuronal transcriptome and cognition of rAAV5 transduction with three different doses in the mouse hippocampus.Frontiers in molecular neuroscience · 2023Article
- Optogenetics in primate cortical networks.Frontiers in neuroanatomy · 2023Article
- Applications of chemogenetics in non-human primates.Current opinion in pharmacology · 2022Review
- Dissecting the Prefrontal Network With Pathway-Selective Manipulation in the Macaque Brain-A Review.Frontiers in neuroscience · 2022Review
- Combining brain perturbation and neuroimaging in non-human primates.NeuroImage · 2021Review
- Dendritic calcium signals in rhesus macaque motor cortex drive an optical brain-computer interface.Nature communications · 2021Article
- Development of optically controlled "living electrodes" with long-projecting axon tracts for a synaptic brain-machine interface.Science advances · 2021Article
- Nonhuman Primate Optogenetics: Current Status and Future Prospects.Advances in experimental medicine and biology · 2021Review
- An Open Resource for Non-human Primate Optogenetics.Neuron · 2020Article
- Gene delivery to neurons in the auditory brainstem of barn owls using standard recombinant adeno-associated virus vectors.Current research in neurobiology · 2020Article
- Histological assessment of optogenetic tools to study fronto-visual and fronto-parietal cortical networks in the rhesus macaque.Scientific reports · 2020Article
- Towards the optical cochlear implant: optogenetic approaches for hearing restoration.EMBO molecular medicine · 2020Review
- Intrathymic adeno-associated virus gene transfer rapidly restores thymic function and long-term persistence of gene-corrected T cells.The Journal of allergy and clinical immunology · 2020Article
- New era of optogenetics: from the central to peripheral nervous system.Critical reviews in biochemistry and molecular biology · 2020Review
- A Step-by-Step Protocol for Optogenetic Kindling.Frontiers in neural circuits · 2020Article
- Behavioral Effect of Chemogenetic Inhibition Is Directly Related to Receptor Transduction Levels in Rhesus Monkeys.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Gene delivery to the primate central nervous system via recombinant adeno-associated viral vectors (AAV) allows neurophysiologists to control and observe neural activity precisely. A current limitation of this approach is variability in vector transduction efficiency. Low levels of transduction can foil experimental manipulations, prompting vector readministration. The ability to make multiple vector injections into the same animal, even in cases where successful vector transduction has already been achieved, is also desirable. However, vector readministration has consequences for humoral immunity and gene delivery that depend on vector dosage and route of administration in complex ways. As part of optogenetic experiments in rhesus monkeys, we analyzed blood sera collected before and after AAV injections into the brain and quantified neutralizing antibodies to AAV using an in vitro assay. We found that injections of AAV1 and AAV9 vectors elevated neutralizing antibody titers consistently. These immune responses were specific to the serotype injected and were long lasting. These results demonstrate that optogenetic manipulations in monkeys trigger immune responses to AAV capsids, suggesting that vector readministration may have a higher likelihood of success by avoiding serotypes injected previously.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.