ArticleACS combinatorial science2017
An Integrated Microfluidic Processor for DNA-Encoded Combinatorial Library Functional Screening.
Article in ACS combinatorial science, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
39 citing papers in PubMed, 95 citations in OpenAlex.
- Automated DNA-Encoded Library Synthesis and Activity-Based Screening at the Attomole Scale.Journal of medicinal chemistry · 2026Article
- Solid-phase DNA-encoded library synthesis: a master builder's instructions.Nature protocols · 2026Review
- DNA-encoded chemical libraries on stage.Nature chemical biology · 2025Article
- DNA-Encoded Peptide Library Synthesis and Applications, a Mini-Review.Methods in molecular biology (Clifton, N.J.) · 2025Review
- My Perspective on OBOC Combinatorial Technology.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Self-Driving Laboratories for Chemistry and Materials Science.Chemical reviews · 2024Review
- Small-molecule discovery through DNA-encoded libraries.Nature reviews. Drug discovery · 2023Review
- Diversity-oriented synthesis encoded by deoxyoligonucleotides.Nature communications · 2023Article
- Liposomal Permeation Assay for Droplet-Scale Pharmacokinetic Screening.Journal of medicinal chemistry · 2023Article
- Translating the Genome into Drugs.Accounts of chemical research · 2023Article
- Study of an RNA-Focused DNA-Encoded Library Informs Design of a Degrader of a r(CUG) Repeat Expansion.Journal of the American Chemical Society · 2022Article
- A comprehensive review on advancements in tissue engineering and microfluidics toward kidney-on-chip.Biomicrofluidics · 2022Review
- Recent advances in DNA-encoded dynamic libraries.RSC chemical biology · 2022Review
- Strategies for developing DNA-encoded libraries beyond binding assays.Nature chemistry · 2022Review
- DNA-Encoded Chemistry: Drug Discovery from a Few Good Reactions.Chemical reviews · 2021Review
- Alkaloids in Contemporary Drug Discovery to Meet Global Disease Needs.Molecules (Basel, Switzerland) · 2021Review
- Scanning Protein Surfaces with DNA-Encoded Libraries.ChemMedChem · 2021Review
- Design, Screening, and Testing of Non-Rational Peptide Libraries with Antimicrobial Activity: In Silico and Experimental Approaches.Antibiotics (Basel, Switzerland) · 2020Review
- Considerations for Achieving Maximized DNA Recovery in Solid-Phase DNA-Encoded Library Synthesis.ACS combinatorial science · 2020Article
- The Medicinal Chemistry in the Era of Machines and Automation: Recent Advances in Continuous Flow Technology.Journal of medicinal chemistry · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
DNA-encoded synthesis is rekindling interest in combinatorial compound libraries for drug discovery and in technology for automated and quantitative library screening. Here, we disclose a microfluidic circuit that enables functional screens of DNA-encoded compound beads. The device carries out library bead distribution into picoliter-scale assay reagent droplets, photochemical cleavage of compound from the bead, assay incubation, laser-induced fluorescence-based assay detection, and fluorescence-activated droplet sorting to isolate hits. DNA-encoded compound beads (10-μm diameter) displaying a photocleavable positive control inhibitor pepstatin A were mixed (1920 beads, 729 encoding sequences) with negative control beads (58 000 beads, 1728 encoding sequences) and screened for cathepsin D inhibition using a biochemical enzyme activity assay. The circuit sorted 1518 hit droplets for collection following 18 min incubation over a 240 min analysis. Visual inspection of a subset of droplets (1188 droplets) yielded a 24% false discovery rate (1166 pepstatin A beads; 366 negative control beads). Using template barcoding strategies, it was possible to count hit collection beads (1863) using next-generation sequencing data. Bead-specific barcodes enabled replicate counting, and the false discovery rate was reduced to 2.6% by only considering hit-encoding sequences that were observed on >2 beads. This work represents a complete distributable small molecule discovery platform, from microfluidic miniaturized automation to ultrahigh-throughput hit deconvolution by sequencing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.