ArticlePlant biotechnology journal2017
Next-generation transgenic cotton: pyramiding RNAi and Bt counters insect resistance.
Article in Plant biotechnology journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 133 citations in OpenAlex.
- De novo transcriptome meta-analysis reveals candidate genes involved in life-stage transitions for RNAi-mediated management of the citrus root weevil (Diaprepes abbreviatus).Pest management science · 2026Article
- Computational Discovery of NPA001937: A Novel Carotenoid Targeting Conserved Insect Proteins for Sustainable Pest Management.Applied biochemistry and biotechnology · 2026Article
- Creating resistance to the whiteflyFrontiers in plant science · 2026Article
- Review
- Assessment of Potential Toxic Effects of RNAi-Based Transgenic Cotton on the Non-Target PredatorBiology · 2025Article
- Article
- Using transcriptome analysis to evaluate the impact ofFrontiers in plant science · 2025Article
- Host-Delivered RNA Interference for Durable Pest Resistance in Plants: Advanced Methods, Challenges, and Applications.Molecular biotechnology · 2024Review
- The new kid on the block in insect pest management: sprayable RNAi goes commercial.Science China. Life sciences · 2024Article
- Biotechnology and Solutions: Insect-Pest-Resistance Management for Improvement and Development of Bt Cotton (Plants (Basel, Switzerland) · 2023Article
- Plant-AssociatedPlants (Basel, Switzerland) · 2023Review
- Review
- Rotation of Multiple Single-Gene Transgenic Crops Did Not Slow the Evolution of Resistance to Cry1F or Cry1Ie inInsects · 2023Article
- Cry1Ba1-mediated toxicity of transgenicFrontiers in insect science · 2023Article
- Overexpression of soybean trypsin inhibitor genes decreases defoliation by corn earworm (Frontiers in plant science · 2023Article
- Stabilized Double-Stranded RNA Strategy Improves Cotton Resistance to CBW (International journal of molecular sciences · 2022Article
- Conceptual framework for the insect metamorphosis from larvae to pupae by transcriptomic profiling, a case study of Helicoverpa armigera (Lepidoptera: Noctuidae).BMC genomics · 2022Article
- RNA interference (RNAi) applications to the management of fall armyworm,Frontiers in molecular biosciences · 2022Review
- Application progress of plant-mediated RNAi in pest control.Frontiers in bioengineering and biotechnology · 2022Review
- Biotechnological Approaches for Host Plant Resistance to Insect Pests.Frontiers in genetics · 2022Review
Corrections and comments
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Authors and funding
22 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transgenic crops producing insecticidal proteins from the bacterium Bacillus thuringiensis (Bt) are extensively cultivated worldwide. To counter rapidly increasing pest resistance to crops that produce single Bt toxins, transgenic plant 'pyramids' producing two or more Bt toxins that kill the same pest have been widely adopted. However, cross-resistance and antagonism between Bt toxins limit the sustainability of this approach. Here we describe development and testing of the first pyramids of cotton combining protection from a Bt toxin and RNA interference (RNAi). We developed two types of transgenic cotton plants producing double-stranded RNA (dsRNA) from the global lepidopteran pest Helicoverpa armigera designed to interfere with its metabolism of juvenile hormone (JH). We focused on suppression of JH acid methyltransferase (JHAMT), which is crucial for JH synthesis, and JH-binding protein (JHBP), which transports JH to organs. In 2015 and 2016, we tested larvae from a Bt-resistant strain and a related susceptible strain of H. armigera on seven types of cotton: two controls, Bt cotton, two types of RNAi cotton (targeting JHAMT or JHBP) and two pyramids (Bt cotton plus each type of RNAi). Both types of RNAi cotton were effective against Bt-resistant insects. Bt cotton and RNAi acted independently against the susceptible strain. In computer simulations of conditions in northern China, where millions of farmers grow Bt cotton as well as abundant non-transgenic host plants of H. armigera, pyramided cotton combining a Bt toxin and RNAi substantially delayed resistance relative to using Bt cotton alone.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.