ArticleJournal of experimental botany2017
Waxy and non-waxy barley cultivars exhibit differences in the targeting and catalytic activity of GBSS1a.
Article in Journal of experimental botany, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- High β-Glucan Whole Grain Barley Reduces Postprandial Glycemic Response in Healthy Adults-Part One of a Randomized Controlled Trial.Nutrients · 2025Trial
- Effects of a novel loss-of-function waxy endosperm allele on sorghum seed development and grain quality.G3 (Bethesda, Md.) · 2025Article
- Functional Characterization of Arabidopsis α-Amylase3 (AMY3): Amylose Specificity and Structural Insights into Its Duplex Carbohydrate-Binding Module.bioRxiv : the preprint server for biology · 2025Article
- Physicochemical Properties of Starch Isolated from Betahealth, a High β-Glucan Barley Cultivar.Foods (Basel, Switzerland) · 2025Article
- Unveiling the genetic architecture of barley embryo: QTL mapping, candidate genes identification and its relationship with kernel size and early vigour.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Identification of the Granule-Bound Starch Synthase (GBSS) Genes Involved in Amylose Biosynthesis in Tartary Buckwheat (Plants (Basel, Switzerland) · 2025Article
- OsLESV and OsESV1 promote transitory and storage starch biosynthesis to determine rice grain quality and yield.Plant communications · 2024Article
- Creating a zero amylose barley with high soluble sugar content by genome editing.Plant molecular biology · 2024Article
- Protein targeting to starch 1, a functional protein of starch biosynthesis in wheat (Triticum aestivum L.).Plant molecular biology · 2022Article
- Article
- Gene Coexpression Network Analysis Indicates that Hub Genes Related to Photosynthesis and Starch Synthesis Modulate Salt Stress Tolerance inInternational journal of molecular sciences · 2021Article
- The Triple Jags of Dietary Fibers in Cereals: How Biotechnology IsFrontiers in plant science · 2021Review
- Fast and Inexpensive Phenotyping and Genotyping Methods for Evaluation of Barley Mutant Population.Plants (Basel, Switzerland) · 2020Article
- Molecular Dispersion of Starch as a Crucial Parameter during Size-Exclusion Chromatography.Foods (Basel, Switzerland) · 2020Article
- Natural Polymorphisms in Arabidopsis Result in Wide Variation or Loss of the Amylose Component of Starch.Plant physiology · 2020Article
- Expression of starch-binding factor CBM20 in barley plastids controls the number of starch granules and the level of CO2 fixation.Journal of experimental botany · 2020Article
- CRISPR/Cas9-Based Mutagenesis of Starch Biosynthetic Genes in Sweet Potato (Ipomoea Batatas) for the Improvement of Starch Quality.International journal of molecular sciences · 2019Article
- Imaging Amyloplasts in the Developing Endosperm of Barley and Rice.Scientific reports · 2019Article
- Protein Targeting to Starch 1 is essential for starchy endosperm development in barley.Journal of experimental botany · 2019Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Amylose synthesis is strictly associated with activity of granule-bound starch synthase (GBSS) enzymes. Among several crops there are cultivars containing starch types with either little or no amylose known as near-waxy or waxy. This (near) amylose-free phenotype is associated with a single locus (waxy) which has been mapped to GBSS-type genes in different crops. Most waxy varieties are a result of either low or no expression of a GBSS gene. However, there are some waxy cultivars where the GBSS enzymes are expressed normally. For these types, single nucleotide polymorphisms have been hypothesized to represent amino-acid substitutions leading to loss of catalytic activity. We here confirm that the HvGBSSIa enzyme from one such waxy barley variety, CDC_Alamo, has a 90% reduction in catalytic activity. We also engineered plants with expression of transgenic C-terminal green fluorescent protein-tagged HvGBSSIa of both the non-waxy type and of the CDC_Alamo type to monitor their subcellular localization patterns in grain endosperm. HvGBSSIa from non-waxy cultivars was found to localize in discrete concentric spheres strictly within starch granules. In contrast, HvGBSSIa from waxy CDC_Alamo showed deficient starch targeting mostly into unknown subcellular bodies of 0.5-3 µm in size, indicating that the waxy phenotype of CDC_Alamo is associated with deficient targeting of HvGBSSIa into starch granules.
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