ArticleJournal of biomedical optics2017
Implementation of fluorescence confocal mosaicking microscopy by "early adopter" Mohs surgeons and dermatologists: recent progress.
Article in Journal of biomedical optics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 38 citations in OpenAlex.
- Machine learning-guided bioelectrical impedance mapping for rapid adjunctive margin assessment in Mohs surgery.npj biomedical innovations · 2026Article
- Intraoperative Margin Control in Eyelid Tumor Surgery: Current Standards, Imaging Advances, and Emerging Techniques.Current oncology (Toronto, Ont.) · 2026Review
- Effects of formalin fixation on ex vivo confocal microscopy imaging: towards rapid pathology.Clinical and experimental dermatology · 2025Article
- Perspective on the use of fluorescence molecular imaging for peripheral and deepJournal of biomedical optics · 2025Review
- Ex Vivo Confocal Microscopy Speeds up Surgical Margin Control of Re-Excised Skin Tumors and Greatly Shortens In-Hospital Stay.Cancers · 2024Article
- Quantitative and comparative assessment of dyes and protocols for rapid ex vivo microscopy of fresh tissues.Scientific reports · 2024Article
- Piston-based specimen holder for rapid surgical and biopsy specimen imaging.Biomedical optics express · 2024Article
- High-resolution full-field optical coherence tomography microscope for the evaluation of freshly excised skin specimens during Mohs surgery: A feasibility study.Journal of biophotonics · 2024Article
- Paired-agent imaging as a rapidFrontiers in oncology · 2023Article
- A Feasibility Study for Immediate Histological Assessment of Various Skin Biopsies Using Ex Vivo Confocal Laser Scanning Microscopy.Diagnostics (Basel, Switzerland) · 2022Article
- Radiodynamic Therapy with Acridine Orange Is an Effective Treatment for Bone Metastases.Biomedicines · 2022Article
- Classification of Basal Cell Carcinoma in Ex Vivo Confocal Microscopy Images from Freshly Excised Tissues Using a Deep Learning Algorithm.The Journal of investigative dermatology · 2022Article
- In vivo microscopy as an adjunctive tool to guide detection, diagnosis, and treatment.Journal of biomedical optics · 2022Article
- Complete visualization of epidermal margin during ex vivo confocal microscopy of excised tissue with 3-dimensional mosaicking and intensity projection.Journal of the American Academy of Dermatology · 2022Article
- An international 3-center training and reading study to assess basal cell carcinoma surgical margins with ex vivo fluorescence confocal microscopy.Journal of cutaneous pathology · 2021Article
- Exploring the utility of Deep Red Anthraquinone 5 for digital staining of ex vivo confocal micrographs of optically sectioned skin.Journal of biophotonics · 2021Article
- Intravital Imaging Techniques for Biomedical and Clinical Research.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2020Review
- Ex Vivo Microscopy: A Promising Next-Generation Digital Microscopy Tool for Surgical Pathology Practice.Archives of pathology & laboratory medicine · 2019Review
- Peri-operative delineation of non-melanoma skin cancer margins in vivo with handheld reflectance confocal microscopy and video-mosaicking.Journal of the European Academy of Dermatology and Venereology : JEADV · 2019Article
- Acridine Orange: A Review of Novel Applications for Surgical Cancer Imaging and Therapy.Frontiers in oncology · 2019Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Confocal mosaicking microscopy (CMM) enables rapid imaging of large areas of fresh tissue ex vivo without the processing that is necessary for conventional histology. When performed in fluorescence mode using acridine orange (nuclear specific dye), it enhances nuclei-to-dermis contrast that enables detection of all types of basal cell carcinomas (BCCs), including micronodular and thin strands of infiltrative types. So far, this technique has been mostly validated in research settings for the detection of residual BCC tumor margins with high sensitivity of 89% to 96% and specificity of 99% to 89%. Recently, CMM has advanced to implementation and testing in clinical settings by “early adopter” Mohs surgeons, as an adjunct to frozen section during Mohs surgery. We summarize the development of CMM guided imaging of ex vivo skin tissues from bench to bedside. We also present its current state of application in routine clinical workflow not only for the assessment of residual BCC margins in the Mohs surgical setting but also for some melanocytic lesions and other skin conditions in clinical dermatology settings. Last, we also discuss the potential limitations of this technology as well as future developments. As this technology advances further, it may serve as an adjunct to standard histology and enable rapid surgical pathology of skin cancers at the bedside.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.