ArticleScientific reports2017
Quick and inexpensive paraffin-embedding method for dynamic bone formation analyses.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
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- KBTBD2 controls bone development by regulating IGF-1 signaling during osteoblast differentiation.Cell death and differentiation · 2025Article
- Protein kinase G2 activation restores Wnt signaling and bone mass in glucocorticoid-induced osteoporosis in mice.JCI insight · 2024Article
- 3'-Sialyllactose alleviates bone loss by regulating bone homeostasis.Communications biology · 2024Article
- Quantitative analysis of trabecular bone tissue cryosections via a fully automated neural network-based approach.PloS one · 2024Article
- Investigating the temporal roles of decorin and biglycan in tendon healing.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2023Article
- An osteoinductive and biodegradable intramedullary implant accelerates bone healing and mitigates complications of bone transport in male rats.Nature communications · 2023Article
- Enhancement of Scaffold In Vivo Biodegradability for Bone Regeneration Using P28 Peptide Formulations.Pharmaceuticals (Basel, Switzerland) · 2023Article
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- Protein Kinase G2 Is Essential for Skeletal Homeostasis and Adaptation to Mechanical Loading in Male but Not Female Mice.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2023Article
- ERα Signaling in a Subset of CXCL12-Abundant Reticular Cells Regulates Trabecular Bone in Mice.JBMR plus · 2022Article
- Heparan sulfate-dependent RAGE oligomerization is indispensable for pathophysiological functions of RAGE.eLife · 2022Article
- Article
- Skeletal stem cell fate defects caused by Pdgfrb activating mutation.Development (Cambridge, England) · 2021Article
- Endothelium-specific depletion of LRP1 improves glucose homeostasis through inducing osteocalcin.Nature communications · 2021Article
- A novel cryo-embedding method for in-depth analysis of craniofacial mini pig bone specimens.Scientific reports · 2020Article
- Protein kinase G1 regulates bone regeneration and rescues diabetic fracture healing.JCI insight · 2020Article
- Mass Spectrometry Imaging (MSI) of Fresh Bones using Infrared Matrix-Assisted Laser Desorption Electrospray Ionization (IR-MALDESI).Analytical methods : advancing methods and applications · 2019Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
We have developed a straightforward method that uses paraffin-embedded bone for undemineralized thin sectioning, which is amenable to subsequent dynamic bone formation measurements. Bone has stiffer material properties than paraffin, and therefore has hereforto usually been embedded in plastic blocks, cured and sectioned with a tungsten carbide knife to obtain mineralized bone sections for dynamic bone formation measures. This process is expensive and requires special equipment, experienced personnel, and time for the plastic to penetrate the bone and cure. Our method utilizes a novel way to prepare mineralized bone that increases its compliance so that it can be embedded and easily section in paraffin blocks. The approach is simple, quick, and costs less than 10% of the price for plastic embedded bone sections. While not effective for static bone measures, this method allows dynamic bone analyses to be readily performed in laboratories worldwide which might not otherwise have access to traditional (plastic) equipment and expertise.
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Registered trials
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