ArticleStem cell reports2017
Healing of a Large Long-Bone Defect through Serum-Free In Vitro Priming of Human Periosteum-Derived Cells.
Article in Stem cell reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
41 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Potential of periosteal cells in bone and cartilage regeneration: a systematic review.Frontiers in bioengineering and biotechnology · 2023Pooled it
- Packed for Ossification: High-Density Bioprinting of hPDC Spheroids in HAMA Toward Endochondral Ossification.Advanced healthcare materials · 2026Article
- Start, Stop, Rewind, Repeat-Cyclic Exposure of Adipose Stromal Cells-derived Cartilage Organoids to Chondrogenic and Proliferative Cues to Achieve Scaled-up and Customizable Bone Formation by Endochondral Ossification.Advanced healthcare materials · 2026Article
- Modern Approaches and Emerging Biological Therapies to Treat Fracture Nonunion.Pharmaceutics · 2025Review
- Single-cell RNA sequencing reveals early cell dynamics of MSC-based therapy in long bone critical-size defects in mice.Journal of orthopaedic translation · 2025Article
- 4D Biofabrication of Magnetically Augmented Callus Assembloid Implants Enables Rapid Endochondral Ossification via Activation of Mechanosensitive Pathways.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Callus organoids reveal distinct cartilage to bone transition mechanisms across donors and a role for biological sex.Bone research · 2025Article
- Treatment of Bone Defects and Nonunion via Novel Delivery Mechanisms, Growth Factors, and Stem Cells: A Review.ACS biomaterials science & engineering · 2024Review
- Role of Adipose-Derived Mesenchymal Stem Cells in Bone Regeneration.International journal of molecular sciences · 2024Review
- Towards Stem Cell Therapy for Critical-Sized Segmental Bone Defects: Current Trends and Challenges on the Path to Clinical Translation.Journal of functional biomaterials · 2024Review
- Cell membrane vesicles derived from hBMSCs and hUVECs enhance bone regeneration.Bone research · 2024Article
- Single-Step Biofabrication of In Situ Spheroid-Forming Compartmentalized Hydrogel for Clinical-Sized Cartilage Tissue Formation.Advanced healthcare materials · 2024Article
- 3D-Printed Osteoinductive Polymeric Scaffolds with Optimized Architecture to Repair a Sheep Metatarsal Critical-Size Bone Defect.Advanced healthcare materials · 2023Article
- Stirred culture of cartilaginous microtissues promotes chondrogenic hypertrophy through exposure to intermittent shear stress.Bioengineering & translational medicine · 2023Article
- The synovial environment steers cartilage deterioration and regeneration.Science advances · 2023Article
- Periosteal Skeletal Stem and Progenitor Cells in Bone Regeneration.Current osteoporosis reports · 2022Review
- Progress of Periosteal Osteogenesis: The Prospect of In Vivo Bioreactor.Orthopaedic surgery · 2022Review
- Advances in Regenerative Sports Medicine Research.Frontiers in bioengineering and biotechnology · 2022Review
- Coculture of Endothelial and Stromal Cells to Promote Concurrent Osteogenesis and Vasculogenesis.Tissue engineering. Part A · 2021Article
- Human pluripotent stem cell-derived cartilaginous organoids promote scaffold-free healing of critical size long bone defects.Stem cell research & therapy · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clinical translation of cell-based strategies for regenerative medicine demands predictable in vivo performance where the use of sera during in vitro preparation inherently limits the efficacy and reproducibility. Here, we present a bioinspired approach by serum-free pre-conditioning of human periosteum-derived cells, followed by their assembly into microaggregates simultaneously primed with bone morphogenetic protein 2 (BMP-2). Pre-conditioning resulted in a more potent progenitor cell population, while aggregation induced osteochondrogenic differentiation, further enhanced by BMP-2 stimulation. Ectopic implantation displayed a cascade of events that closely resembled the natural endochondral process resulting in bone ossicle formation. Assessment in a critical size long-bone defect in immunodeficient mice demonstrated successful bridging of the defect within 4 weeks, with active contribution of the implanted cells. In short, the presented serum-free process represents a biomimetic strategy, resulting in a cartilage tissue intermediate that, upon implantation, robustly leads to the healing of a large long-bone defect.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.