ArticleMolecular microbiology2017
Systematic genetic dissection of PTS in Vibrio cholerae uncovers a novel glucose transporter and a limited role for PTS during infection of a mammalian host.
Article in Molecular microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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31 citing papers in PubMed, 60 citations in OpenAlex.
- Bifidobacterium lactis Probio-M8 improves bone metabolism in patients with postmenopausal osteoporosis, possibly by modulating the gut microbiota.European journal of nutrition · 2023Trial
- Actuation of CRP activating region 3 by formylation modulates theProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Article
- HPr prevents FruR-mediated facilitation of RNA polymerase binding to the fru promoter in Vibrio cholerae.Nucleic acids research · 2023Article
- A simple mechanism for integration of quorum sensing and cAMP signalling inbioRxiv : the preprint server for biology · 2023Article
- Disrupting Central Carbon Metabolism Increases β-Lactam Antibiotic Susceptibility in Vibrio cholerae.Journal of bacteriology · 2023Article
- The Role of Nutrients and Nutritional Signals in the Pathogenesis of Vibrio cholerae.Advances in experimental medicine and biology · 2023Article
- Advances in cholera research: from molecular biology to public health initiatives.Frontiers in microbiology · 2023Review
- Article
- A mannose-sensing AraC-type transcriptional activator regulates cell-cell aggregation of Vibrio cholerae.NPJ biofilms and microbiomes · 2022Article
- Gut Microbial Divergence Between Three Hadal Amphipod Species from the Isolated Hadal Trenches.Microbial ecology · 2022Article
- A Vibrio-based microbial platform for accelerated lignocellulosic sugar conversion.Biotechnology for biofuels and bioproducts · 2022Article
- Vibrio cholerae requires oxidative respiration through the bd-I and cbb3 oxidases for intestinal proliferation.PLoS pathogens · 2022Article
- Animal models for dissecting Vibrio cholerae intestinal pathogenesis and immunity.Current opinion in microbiology · 2022Review
- A dual-function RNA balances carbon uptake and central metabolism in Vibrio cholerae.The EMBO journal · 2021Article
- A fructose/HNature communications · 2021Article
- Cra and cAMP Receptor Protein Have Opposing Roles in the Regulation ofJournal of bacteriology · 2021Article
- Control of Competence in Vibrio fischeri.Applied and environmental microbiology · 2021Article
- Vibrio cholerae FruR facilitates binding of RNA polymerase to the fru promoter in the presence of fructose 1-phosphate.Nucleic acids research · 2021Article
- Metagenomic Insights Into the Structure and Function of Intestinal Microbiota of the Hadal Amphipods.Frontiers in microbiology · 2021Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
A common mechanism for high affinity carbohydrate uptake in microbial species is the phosphoenolpyruvate-dependent phosphotransferase system (PTS). This system consists of a shared component, EI, which is required for all PTS transport, and numerous carbohydrate uptake transporters. In Vibrio cholerae, there are 13 distinct PTS transporters. Due to genetic redundancy within this system, the carbohydrate specificity of each of these transporters is not currently defined. Here, using multiplex genome editing by natural transformation (MuGENT), we systematically dissect PTS transport in V. cholerae. Specifically, we generated a mutant strain that lacks all 13 PTS transporters, and from this strain, we created a panel of mutants where each expresses a single transporter. Using this panel, we have largely defined the carbohydrate specificities of each PTS transporter. In addition, this analysis uncovered a novel glucose transporter. We have further defined the mechanism of this transporter and characterized its regulation. Using our 13 PTS transporter mutant, we also provide the first clear evidence that carbohydrate transport by the PTS is not essential during infection in an infant mouse model of cholera. In summary, this study shows how multiplex genome editing can be used to rapidly dissect complex biological systems and genetic redundancy in microbial systems.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.