ArticlePloS one2017
De novo assembly, characterization and annotation for the transcriptome of Sarcocheilichthys sinensis.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Chromosome-Level Genome Assembly and Annotation of the Chinese Lizard Gudgeon (Animals : an open access journal from MDPI · 2026Article
- Article
- Cross-species Exon Capture and Whole Exome Sequencing: Application, Utility and Challenges for Genomic Resource Development in Non-model Species.Marine biotechnology (New York, N.Y.) · 2021Article
- Polymorphisms of the growth hormone gene and their association with growth traits and sex in Sarcocheilichthys sinensis.Molecular genetics and genomics : MGG · 2020Article
- Characterization and phylogenetic analysis of the mitochondrial genome ofMitochondrial DNA. Part B, Resources · 2020Article
- Characterization and analysis of the transcriptome in Gymnocypris selincuoensis on the Qinghai-Tibetan Plateau using single-molecule long-read sequencing and RNA-seq.DNA research : an international journal for rapid publication of reports on genes and genomes · 2019Article
- Article
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Authors and funding
6 authors.
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Abstract
The Chinese lake gudgeon Sarcocheilichthys sinensis is a small cyprinid fish with great aquaculture potential both for its edible and ornamental values. Nevertheless, available genomic and transcriptomic information for this fish is extremely deficient. In this study, a normalized cDNA library was constructed using 13 mixed tissues of an adult male S. sinensis, and was sequenced by the Illumina HiSeq2500 platform. De novo assembly was performed using 38,911,511 obtained clean reads, and a total of 147,282 unigenes with an average length of 900 bp were finally achieved. 96.2% of these unigenes were annotated in 9 public databases, and 16 segments of growth-related genes were identified for future studies. In addition, 28,493 unigenes were assigned to 61 subcategories of Gene Ontology (GO), and 10,483 unigenes were assigned to 25 categories of Cluster of Orthologous Group (COG). Moreover, 14,943 unigenes were classified into 225 pathways of the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. A total of 30,666 microsatellites were detected from 17,627 unigenes with an average distribution density of 1:2405 bp. This transcriptome data set will be valuable for researches on discovery, expression and evolution on genes of interest. Meanwhile, the identified microsatellites would be useful tools for genetic and genomic studies in S. sinensis.
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