ArticlePloS one2017
Transcriptomic, cellular and life-history responses of Daphnia magna chronically exposed to benzotriazoles: Endocrine-disrupting potential and molting effects.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 70 citations in OpenAlex.
- Spontaneous Mutations Occur More in Highly Transcribed Regions in Daphnia.Genome biology and evolution · 2026Article
- The Fate of Contaminants of Emerging Concern in an Upflow Anaerobic Sludge Blanket Reactor Coupled with Constructed Wetlands for Decentralized Domestic Wastewater Treatment.Molecules (Basel, Switzerland) · 2025Article
- Daphnia as a model organism to probe biological responses to nanomaterials-from individual to population effects via adverse outcome pathways.Frontiers in toxicology · 2023Review
- Developmental and Reproductive Impacts of Four Bisphenols inInternational journal of molecular sciences · 2022Article
- Using the Daphnia magna Transcriptome to Distinguish Water Source: Wetland and Stormwater Case Studies.Environmental toxicology and chemistry · 2022Article
- Draft genomes for one Microcystis-resistant and one Microcystis-sensitive strain of the water flea, Daphnia pulicaria.G3 (Bethesda, Md.) · 2021Article
- Mode of action evaluation for reduced reproduction in Daphnia pulex exposed to the insensitive munition, 1-methyl-3-nitro-1-nitroguanidine (MeNQ).Ecotoxicology (London, England) · 2021Article
- Greater toxic potency of bisphenol AF than bisphenol A in growth, reproduction, and transcription of genes in Daphnia magna.Environmental science and pollution research international · 2021Article
- Article
- Different as night and day: Behavioural and life history responses to varied photoperiods in Daphnia magna.Molecular ecology · 2019Article
- A major release of urban untreated wastewaters in the St. Lawrence River (Quebec, Canada) altered growth, reproduction, and redox status in experimentally exposed Daphnia magna.Ecotoxicology (London, England) · 2019Article
- Differential gene transcription across the life cycle in Daphnia magna using a new all genome custom-made microarray.BMC genomics · 2018Article
- Identification of General Patterns of Sex-Biased Expression inG3 (Bethesda, Md.) · 2018Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Benzotriazoles (BZTs) are ubiquitous aquatic contaminants used in a wide range of industrial and domestic applications from aircraft deicers to dishwasher tablets. Acute toxicity has been reported in aquatic organisms for some of the BZTs but their mode of action remains unknown. The objectives of this study were to evaluate the transcriptomic response of D. magna exposed to sublethal doses of 1H-benzotriazole (BTR), 5-methyl-1H-benzotriazole (5MeBTR) and 5-chloro-1H-benzotriazole (5ClBTR) using RNA-sequencing and quantitative real-time PCR. Cellular and life-history endpoints (survival, number of neonates, growth) were also investigated. Significant effects on the molting frequency were observed after 21-d exposure to 5MeBTR and 5ClBTR. No effects on molting frequency were observed for BTR but RNA-seq results indicated that this BZT induced the up-regulation of genes coding for cuticular proteins, which could have compensated the molting disruption. Molting in cladocerans is actively controlled by ecdysteroid hormones. Complementary short-term temporal analysis (4- and 8-d exposure) of the transcription of genes related to molting and hormone-mediated processes indicated that the three compounds had specific modes of action. BTR induced the transcription of genes involved in 20-hydroxyecdysone synthesis, which suggests pro-ecdysteroid properties. 5ClBTR exposure induced protein activity and transcriptional levels of chitinase enzymes, associated with an impact on ecdysteroid signaling pathways, which could explain the decrease in molt frequency. Finally, 5MeBTR seemed to increase molt frequency through epigenetic processes. Overall, results suggested that molting effects observed at the physiological level could be linked to endocrine regulation impacts of BZTs at the molecular level.
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