ArticleACS chemical biology2017
The GCaMP-R Family of Genetically Encoded Ratiometric Calcium Indicators.
Article in ACS chemical biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 74 citations in OpenAlex.
- Advances in Imaging of Plant CaBiomolecules · 2026Review
- Embryonic Avian Skin Explant Cultures: A Versatile Model for Investigating Tissue Patterning Mechanisms.The Journal of investigative dermatology · 2025Review
- The Multifaceted Role of Calcium Signaling in Regulated Necrosis.Biomolecules · 2025Review
- Integration of signal transduction pathways in sensory epithelial enteroendocrine cells.Molecular biology of the cell · 2025Review
- CaMK modulates sensory neural activity to control longevity and proteostasis.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- A Genetically Encoded Fluorescent Biosensor for Intracellular Measurement of Malonyl-CoA.ACS bio & med chem Au · 2025Article
- Calcium Indicators with Fluorescence Lifetime-Based Signal Readout: A Structure-Function Study.International journal of molecular sciences · 2024Article
- Article
- A Genetically Encoded Fluorescent Biosensor for Intracellular Measurement of Malonyl-CoA.bioRxiv : the preprint server for biology · 2024Article
- Neurovascular coupling and CONature communications · 2024Article
- Dendrite intercalation between epidermal cells tunes nociceptor sensitivity to mechanical stimuli in Drosophila larvae.PLoS genetics · 2024Article
- Calcium imaging: a technique to monitor calcium dynamics in biological systems.Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Review
- Dendrite intercalation between epidermal cells tunes nociceptor sensitivity to mechanical stimuli inbioRxiv : the preprint server for biology · 2023Article
- Monitoring nutrients in plants with genetically encoded sensors: achievements and perspectives.Plant physiology · 2023Review
- Ratiometric measurement of MAM CaNature communications · 2023Article
- Subcellular Imaging of Neuronal Calcium Handling In Vivo.Journal of visualized experiments : JoVE · 2023Article
- The β-cell primary cilium is an autonomous Ca2+ compartment for paracrine GABA signaling.The Journal of cell biology · 2023Article
- Review
- Absolute measurement of cellular activities using photochromic single-fluorophore biosensors and intermittent quantification.Nature communications · 2022Article
- A change of heart: new roles for cilia in cardiac development and disease.Nature reviews. Cardiology · 2022Review
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
Abstract
We report on GCaMP-Rs, a new family of genetically encoded ratiometric calcium indicators that extend the virtues of the GCaMP proteins to ratiometric measurements. We have engineered a tandem construct of calcium-dependent GCaMP and calcium-independent mCherry fluorescent proteins. The tandem design assures that the two proteins localize in the same cellular compartment(s) and facilitates pixelwise ratiometric measurements; however, Förster resonance energy transfer (FRET) between the fluorophores reduces brightness of the sensor by up to half (depending on the GCaMP variant). To eliminate FRET, we introduced a rigid α-helix, the ER/K helix, between GCaMP and mCherry. Avoiding FRET significantly increases the brightness (notably, even at low calcium concentrations), the signal-to-noise ratio, and the dynamic range.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.