ArticleThe ISME journal2017
Cultivation and characterization of Candidatus Nitrosocosmicus exaquare, an ammonia-oxidizing archaeon from a municipal wastewater treatment system.
Article in The ISME journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 79 papers.
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79 citing papers in PubMed.
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- Interpretable multi-omics machine learning reveals drought-driven shifts in plant-microbe interactions.Environmental microbiome · 2026Article
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- Environmental distribution, physiology and genomic adaptations of arctic ammonia-oxidizing archaea.Frontiers in microbiology · 2026Article
- Historic mine waste contains diverse microbial communities that reflect waste type and geochemistry.Applied and environmental microbiology · 2025Article
- Nitrosarchaeum haohaiensis sp. Nov. CL1Environmental microbiology reports · 2025Article
- Genomic profiling of soil nitrifying microorganisms enriched on floating membrane filter.Journal of microbiology (Seoul, Korea) · 2025Article
- Biofilm lifestyle across different lineages of ammonia-oxidizing archaea.The ISME journal · 2025Article
- Spatiotemporal and temperature-dependent disconnect between ammonia oxidation and dark DIC fixation in deep oligotrophic Lake Constance.ISME communications · 2025Article
- Microbial community succession of home aquarium biofilters associated with early establishment of comammoxISME communications · 2025Article
- Nitrifying Communities in Biological Nitrogen Removal Processes at Tropical Municipal Wastewater Treatment Plants.Microbes and environments · 2025Article
- Predicting gene distribution in ammonia-oxidizing archaea using phylogenetic signals.ISME communications · 2025Article
- Genome-scale metabolic modelling reveals interactions and key roles of symbiont clades in a sponge holobiont.Nature communications · 2024Article
- "mSphere · 2024Article
- Adaptive traits ofmBio · 2024Article
- The Saint-Leonard Urban Glaciotectonic Cave Harbors Rich and Diverse Planktonic and Sedimentary Microbial Communities.Microorganisms · 2024Article
- Plants Restoration Drives the Gobi Soil Microbial Diversity for Improving Soil Quality.Plants (Basel, Switzerland) · 2024Article
- ComammoxApplied and environmental microbiology · 2024Article
- Metabolic potential of Nitrososphaera-associated clades.The ISME journal · 2024Article
19 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
7 authors.
Funding
Abstract
Thaumarchaeota have been detected in several industrial and municipal wastewater treatment plants (WWTPs), despite the fact that ammonia-oxidizing archaea (AOA) are thought to be adapted to low ammonia environments. However, the activity, physiology and metabolism of WWTP-associated AOA remain poorly understood. We report the cultivation and complete genome sequence of Candidatus Nitrosocosmicus exaquare, a novel AOA representative from a municipal WWTP in Guelph, Ontario (Canada). In enrichment culture, Ca. N. exaquare oxidizes ammonia to nitrite stoichiometrically, is mesophilic, and tolerates at least 15 mm of ammonium chloride or sodium nitrite. Microautoradiography (MAR) for enrichment cultures demonstrates that Ca. N. exaquare assimilates bicarbonate in association with ammonia oxidation. However, despite using inorganic carbon, the ammonia-oxidizing activity of Ca. N. exaquare is greatly stimulated in enrichment culture by the addition of organic compounds, especially malate and succinate. Ca. N. exaquare cells are coccoid with a diameter of ~1-2 μm. Phylogenetically, Ca. N. exaquare belongs to the Nitrososphaera sister cluster within the Group I.1b Thaumarchaeota, a lineage which includes most other reported AOA sequences from municipal and industrial WWTPs. The 2.99 Mbp genome of Ca. N. exaquare encodes pathways for ammonia oxidation, bicarbonate fixation, and urea transport and breakdown. In addition, this genome encodes several key genes for dealing with oxidative stress, including peroxidase and catalase. Incubations of WWTP biofilm demonstrate partial inhibition of ammonia-oxidizing activity by 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide (PTIO), suggesting that Ca. N. exaquare-like AOA may contribute to nitrification in situ. However, CARD-FISH-MAR showed no incorporation of bicarbonate by detected Thaumarchaeaota, suggesting that detected AOA may incorporate non-bicarbonate carbon sources or rely on an alternative and yet unknown metabolism.
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