ArticleMolecular biotechnology2017
RNAi-Mediated Simultaneous Resistance Against Three RNA Viruses in Potato.
Article in Molecular biotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 67 citations in OpenAlex.
- Plant Genetic Engineering: Technological Pathways, Application Scenarios, and Future Directions.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Enhanced Soybean Immunity to the Soybean Mosaic Virus Through RNA Interference Targeting thePlants (Basel, Switzerland) · 2026Article
- Screening Various Bacterial-Produced Double-Stranded RNAs for Managing Asian Soybean Rust Disease Caused byPlants (Basel, Switzerland) · 2026Article
- Article
- RNA silencing: the future potential strategy for engineering virus resistance in plants.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Review
- The genomic region matters when synthesizing dsRNA for plant virus suppression via RNAi.Virology journal · 2025Article
- RNAi and genome editing of sugarcane: Progress and prospects.The Plant journal : for cell and molecular biology · 2025Review
- Spray-induced gene silencing for crop protection: recent advances and emerging trends.Frontiers in plant science · 2025Review
- Research Progress on Viruses ofBiology · 2024Review
- Detection of exogenous siRNA inside sweet corn bundle sheath cells and the RNAi dynamics in the early stage ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2024Article
- Modern Breeding Strategies and Tools for Durable Late Blight Resistance in Potato.Plants (Basel, Switzerland) · 2024Review
- Direct dsRNA preparation by promoter-free RCT and RNase H cleavage using one circular dsDNA template with a mismatched bubble.RNA (New York, N.Y.) · 2023Article
- Article
- Strategies for Engineering Virus Resistance in Potato.Plants (Basel, Switzerland) · 2023Review
- Generation of Asynaptic Mutants in Potato by DisruptingLife (Basel, Switzerland) · 2023Article
- SilencingPotato research · 2023Article
- Review
- Genome editing (CRISPR-Cas)-mediated virus resistance in potato (Solanum tuberosum L.).Molecular biology reports · 2022Review
- Tomato brown rugose fruit virus: An emerging and rapidly spreading plant RNA virus that threatens tomato production worldwide.Molecular plant pathology · 2022Review
- Comparative evaluation of resistance to potato virus Y (PVY) in three different RNAi-based transgenic potato plants.Transgenic research · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA interference (RNAi) technology has been successfully applied in stacking resistance against viruses in numerous crop plants. During RNAi, the production of small interfering RNAs (siRNAs) from template double-standard RNA (dsRNA) derived from expression constructs provides an on-switch for triggering homology-based targeting of cognate viral transcripts, hence generating a pre-programmed immunity in transgenic plants prior to virus infection. In the current study, transgenic potato lines (Solanum tuberosum cv. Desiree) were generated, expressing fused viral coat protein coding sequences from Potato virus X (PVX), Potato virus Y (PVY), and Potato virus S (PVS) as a 600-bp inverted repeat expressed from a constitutive 35S promoter. The expression cassette (designated Ec1/p5941) was designed to generate dsRNAs having a hairpin loop configuration. The transgene insertions were confirmed by glufosinate resistance, gene-specific PCR, and Southern blotting. Regenerated lines were further assayed for resistance to virus inoculation for up to two consecutive crop seasons. Nearly 100% resistance against PVX, PVY, and PVS infection was observed in transgenic lines when compared with untransformed controls, which developed severe viral disease symptoms. These results establish the efficacy of RNAi using the coat protein gene as a potential target for the successful induction of stable antiviral immunity in potatoes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.