ArticleProceedings of the National Academy of Sciences of the United States of America2017
Strong signal increase in STED fluorescence microscopy by imaging regions of subdiffraction extent.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 114 citations in OpenAlex.
- Breaking the Diffraction Limit: Progress and Prospects of Super-Resolution Fluorescence Microscopy in Biomedical and Materials Research.Journal of fluorescence · 2026Review
- Permeability-Based Measurement of Nanobody Binding to Nuclear Pore Complexes by Transient Scanning Electrochemical Microscopy.Analytical chemistry · 2026Article
- Live-cell STED microscopy enables 50 nm resolution imaging with preserved cell proliferation.Scientific reports · 2026Article
- Integrating the Contrasting Perspectives Between the Constrained Disorder Principle and Deterministic Optical Nanoscopy: Enhancing Information Extraction from Imaging of Complex Systems.Bioengineering (Basel, Switzerland) · 2026Article
- A flexible framework for automated STED super-resolution microscopy.Scientific reports · 2026Article
- Adaptive fluorescence lifetime imaging with per-pixel signal optimization and flexible scanning.Biomedical optics express · 2025Article
- Super-resolution radial fluctuations (SRRF): a versatile and accessible tool for live-cell nanoscopy.Histochemistry and cell biology · 2025Review
- Live-cell imaging powered by computation.Nature reviews. Molecular cell biology · 2024Review
- Pushing the Resolution Limit of Stimulated Emission Depletion Optical Nanoscopy.International journal of molecular sciences · 2023Review
- Optical microscopy gets down to angstroms.Nature biotechnology · 2023Article
- MINSTED nanoscopy enters the Ångström localization range.Nature biotechnology · 2023Article
- Expansion microscopy of neutrophil nuclear structure and extracellular traps.Biophysical reports · 2023Article
- High-throughput line-illumination Raman microscopy with multislit detection.Biomedical optics express · 2023Article
- Focus image scanning microscopy for sharp and gentle super-resolved microscopy.Nature communications · 2022Article
- Sparse deconvolution improves the resolution of live-cell super-resolution fluorescence microscopy.Nature biotechnology · 2022Article
- Technological advances in super-resolution microscopy to study cellular processes.Molecular cell · 2022Review
- Correlative nanophotonic approaches to enlighten the nanoscale dynamics of living cell membranes.Biochemical Society transactions · 2021Review
- 3D test sample for the calibration and quality control of stimulated emission depletion (STED) and confocal microscopes.Communications biology · 2021Article
- MINSTED fluorescence localization and nanoscopy.Nature photonics · 2021Article
- Tomographic STED microscopy.Biomedical optics express · 2020Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Photobleaching remains a limiting factor in superresolution fluorescence microscopy. This is particularly true for stimulated emission depletion (STED) and reversible saturable/switchable optical fluorescence transitions (RESOLFT) microscopy, where adjacent fluorescent molecules are distinguished by sequentially turning them off (or on) using a pattern of light formed as a doughnut or a standing wave. In sample regions where the pattern intensity reaches or exceeds a certain threshold, the molecules are essentially off (or on), whereas in areas where the intensity is lower, that is, around the intensity minima, the molecules remain in the initial state. Unfortunately, the creation of on/off state differences on subdiffraction scales requires the maxima of the intensity pattern to exceed the threshold intensity by a large factor that scales with the resolution. Hence, when recording an image by scanning the pattern across the sample, each molecule in the sample is repeatedly exposed to the maxima, which exacerbates bleaching. Here, we introduce MINFIELD, a strategy for fundamentally reducing bleaching in STED/RESOLFT nanoscopy through restricting the scanning to subdiffraction-sized regions. By safeguarding the molecules from the intensity of the maxima and exposing them only to the lower intensities (around the minima) needed for the off-switching (on-switching), MINFIELD largely avoids detrimental transitions to higher molecular states. A bleaching reduction by up to 100-fold is demonstrated. Recording nanobody-labeled nuclear pore complexes in
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.