ArticleJournal of cell science2017
Error-prone meiotic division and subfertility in mice with oocyte-conditional knockdown of pericentrin.
Article in Journal of cell science, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
37 citing papers in PubMed, 61 citations in OpenAlex.
- Power-law scaling of mitotic spindles with genome sizes across eukaryotes is driven by chromosome crowding.Nature cell biology · 2026Article
- Article
- A human CEP120 gene variant impairs meiotic spindle building causing aneuploidy†.Biology of reproduction · 2026Article
- Fertilization time and oocyte spindle integrity: implication for embryo ploidy.Reproduction & fertility · 2026Article
- Genome-Wide Association Study of Daughter Pregnancy Rate in Crossbred Dairy Cows.International journal of molecular sciences · 2025Article
- Spindle-localized F-actin regulates polar MTOC organization and the fidelity of meiotic spindle formation.Nature communications · 2025Article
- Roles of Tubulin Concentration during Prometaphase and Ran-GTP during Anaphase ofLife science alliance · 2024Article
- Spatio-temporal requirements of Aurora kinase A in mouse oocyte meiotic spindle building.iScience · 2024Article
- Roles of Tubulin Concentration during Prometaphase and Ran-GTP during Anaphase ofbioRxiv : the preprint server for biology · 2024Article
- How Do Environmental Toxicants Affect Oocyte Maturation Via Oxidative Stress?Advances in anatomy, embryology, and cell biology · 2024Review
- Pericentric major satellite transcription is essential for meiotic chromosome stability and spindle pole organization.Open biology · 2023Article
- PAK1 Is Involved in the Spindle Assembly during the First Meiotic Division in Porcine Oocytes.International journal of molecular sciences · 2023Article
- Phosphorylation of adducin-1 by TPX2 promotes interpolar microtubule homeostasis and precise chromosome segregation in mouse oocytes.Cell & bioscience · 2022Article
- Frequent Spindle Assembly Errors Require Structural Rearrangement to Complete Meiosis inInternational journal of molecular sciences · 2022Article
- Acentriolar spindle assembly in mammalian female meiosis and the consequences of its perturbations on human reproduction†.Biology of reproduction · 2022Review
- Microtubule organizing centers regulate spindle positioning in mouse oocytes.Developmental cell · 2022Article
- Azoxystrobin exposure impairs meiotic maturation by disturbing spindle formation in mouse oocytes.Frontiers in cell and developmental biology · 2022Article
- Loss of acentriolar MTOCs disrupts spindle pole Aurora A and assembly of the liquid-like meiotic spindle domain in oocytes.Journal of cell science · 2021Article
- Aurora kinase A is essential for meiosis in mouse oocytes.PLoS genetics · 2021Article
- Centriole and PCM cooperatively recruit CEP192 to spindle poles to promote bipolar spindle assembly.The Journal of cell biology · 2021Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Mouse oocytes lack canonical centrosomes and instead contain unique acentriolar microtubule-organizing centers (aMTOCs). To test the function of these distinct aMTOCs in meiotic spindle formation, pericentrin (Pcnt), an essential centrosome/MTOC protein, was knocked down exclusively in oocytes by using a transgenic RNAi approach. Here, we provide evidence that disruption of aMTOC function in oocytes promotes spindle instability and severe meiotic errors that lead to pronounced female subfertility. Pcnt-depleted oocytes from transgenic (Tg) mice were ovulated at the metaphase-II stage, but show significant chromosome misalignment, aneuploidy and premature sister chromatid separation. These defects were associated with loss of key Pcnt-interacting proteins (γ-tubulin, Nedd1 and Cep215) from meiotic spindle poles, altered spindle structure and chromosome-microtubule attachment errors. Live-cell imaging revealed disruptions in the dynamics of spindle assembly and organization, together with chromosome attachment and congression defects. Notably, spindle formation was dependent on Ran GTPase activity in Pcnt-deficient oocytes. Our findings establish that meiotic division is highly error-prone in the absence of Pcnt and disrupted aMTOCs, similar to what reportedly occurs in human oocytes. Moreover, these data underscore crucial differences between MTOC-dependent and -independent meiotic spindle assembly.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.