ArticleBMC biotechnology2017
Automated freeze-thaw cycles for decellularization of tendon tissue - a pilot study.
Article in BMC biotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 78 citations in OpenAlex.
- Application of ECIS to Evaluate the Effects of Porcine Urinary Bladder Matrix Hydrogels on Caco-2 Cell Attachment, Migration, and Barrier Formation.Gels (Basel, Switzerland) · 2026Article
- Advances in Decellularization of Fish Wastes for Extracellular Matrix Extraction in Sustainable Tissue Engineering and Regenerative Medicine.Bioengineering (Basel, Switzerland) · 2026Review
- Decellularization method and tissue region impact the structural properties and composition of porcine vaginal extracellular matrix.Acta biomaterialia · 2026Article
- Tissue-Cultured Chondrocytes Survive After Irradiation in 1300 Gy Dose.Biomedicines · 2025Article
- Research Progress on the Preparation and Application of Decellularized Tendons.Current issues in molecular biology · 2025Review
- Recent advances in bionic scaffolds for cartilage tissue engineering.Frontiers in bioengineering and biotechnology · 2025Review
- Pathologically altered articular cartilage attracts intense chondrocyte invasion into the extracellular matrix: in vitro pilot study.Knee surgery & related research · 2024Article
- Cartilage Laser Engraving for Fast-Track Tissue Engineering of Auricular Grafts.International journal of molecular sciences · 2024Article
- Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities.Bioactive materials · 2024Review
- Swim bladder-derived biomaterials: structures, compositions, properties, modifications, and biomedical applications.Journal of nanobiotechnology · 2024Review
- Extracellular matrix-derived materials for tissue engineering and regenerative medicine: A journey from isolation to characterization and application.Bioactive materials · 2024Review
- The trend of allogeneic tendon decellularization: literature review.Cell and tissue banking · 2024Review
- Decellularization and Their Significance for Tissue Regeneration in the Era of 3D Bioprinting.ACS omega · 2024Review
- Tailoring the multiscale mechanics of tunable decellularized extracellular matrix (dECM) for wound healing through immunomodulation.Bioactive materials · 2023Article
- Review
- Different Decellularization Methods in Bovine Lung Tissue Reveals Distinct Biochemical Composition, Stiffness, and Viscoelasticity in Reconstituted Hydrogels.ACS applied bio materials · 2023Article
- The Canine Pancreatic Extracellular Matrix in Diabetes Mellitus and Pancreatitis: Its Essential Role and Therapeutic Perspective.Animals : an open access journal from MDPI · 2023Review
- Functional acellular matrix for tissue repair.Materials today. Bio · 2023Review
- Automated Assessment of Cell Infiltration and Removal in Decellularized Scaffolds - Experimental Study in Rabbits.Revista brasileira de ortopedia · 2022Article
- Detergent-Free Decellularization Preserves the Mechanical and Biological Integrity of Murine Tendon.Tissue engineering. Part C, Methods · 2022Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDecellularization of tendon tissue plays a pivotal role in current tissue engineering approaches for in vitro research as well as for translation of graft-based tendon restoration into clinics. Automation of essential decellularization steps like freeze-thawing is crucial for the development of more standardized decellularization protocols and commercial graft production under good manufacturing practice (GMP) conditions in the future.
methodsIn this study, a liquid nitrogen-based controlled rate freezer was utilized for automation of repeated freeze-thawing for decellularization of equine superficial digital flexor tendons. Additional tendon specimens underwent manually performed freeze-thaw cycles based on an established procedure. Tendon decellularization was completed by using non-ionic detergent treatment (Triton X-100). Effectiveness of decellularization was assessed by residual nuclei count and calculation of DNA content. Cytocompatibility was evaluated by culturing allogeneic adipose tissue-derived mesenchymal stromal cells on the tendon scaffolds.
resultsThere were no significant differences in decellularization effectiveness between samples decellularized by the automated freeze-thaw procedure and samples that underwent manual freeze-thaw cycles. Further, we inferred no significant differences in the effectiveness of decellularization between two different cooling and heating rates applied in the automated freeze-thaw process. Both the automated protocols and the manually performed protocol resulted in roughly 2% residual nuclei and 13% residual DNA content. Successful cell culture was achieved with samples decellularized by automated freeze-thawing as well as with tendon samples decellularized by manually performed freeze-thaw cycles.
conclusionsAutomated freeze-thaw cycles performed by using a liquid nitrogen-based controlled rate freezer were as effective as previously described manual freeze-thaw procedures for decellularization of equine superficial digital flexor tendons. The automation of this key procedure in decellularization of large tendon samples is an important step towards the processing of large sample quantities under standardized conditions. Furthermore, with a view to the production of commercially available tendon graft-based materials for application in human and veterinary medicine, the automation of key procedural steps is highly required to develop manufacturing processes under GMP conditions.
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