Evidence map›Paper›PMID 28193191›Full record

ArticleBMC infectious diseases2017

Effective inhibition of MERS-CoV infection by resveratrol.

Shih-Chao Lin, Chi-Tang Ho, Wen-Ho Chuo, Shiming Li, Tony T Wang, Chi-Chen Lin

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC infectious diseases, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 210 papers, 6 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
210citing papers in PubMed, 6 pooled it
18.2field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

210 citing papers in PubMed, 6 syntheses or guidelines pooled it, 362 citations in OpenAlex.

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  13. The Antiviral Activity of Polyphenols.Molecular nutrition & food research · 2025
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150 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 6 institutions in 3 countries.

Shih-Chao LinPh.D. Program in Medical Biotechnology, National Chung Hsing University, Taichung, Taiwan.
Chi-Tang HoDepartment of Food Science, Rutgers University, New Brunswick, New Jersey, USA.
Wen-Ho ChuoDepartment of Pharmacy, Tajen University, Pingtung, Taiwan.
Shiming LiHubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, Huanggang Normal University, Hubei, China.
Tony T WangCenter for Infectious Diseases, Discovery Biology, SRI International, Harrisonburg, VA, 22802, USA. tony.wang@sri.com.
Chi-Chen LinPh.D. Program in Medical Biotechnology, National Chung Hsing University, Taichung, Taiwan. lincc@dragon.nchu.edu.tw.
Asia University · TWHuanggang Normal University · CNNational Chung Hsing University · TWRutgers, The State University of New Jersey · USSRI International · USTajen University · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMiddle East Respiratory Syndrome coronavirus (MERS-CoV) is an emerging viral pathogen that causes severe morbidity and mortality. Up to date, there is no approved or licensed vaccine or antiviral medicines can be used to treat MERS-CoV-infected patients. Here, we analyzed the antiviral activities of resveratrol, a natural compound found in grape seeds and skin and in red wine, against MERS-CoV infection.

methodsWe performed MTT and neutral red uptake assays to assess the survival rates of MERS-infected Vero E6 cells. In addition, quantitative PCR, western blotting, and immunofluorescent assays determined the intracellular viral RNA and protein expression. For viral productivity, we utilized plaque assays to confirm the antiviral properties of resveratrol against MERS-CoV.

resultsResveratrol significantly inhibited MERS-CoV infection and prolonged cellular survival after virus infection. We also found that the expression of nucleocapsid (N) protein essential for MERS-CoV replication was decreased after resveratrol treatment. Furthermore, resveratrol down-regulated the apoptosis induced by MERS-CoV in vitro. By consecutive administration of resveratrol, we were able to reduce the concentration of resveratrol while achieving inhibitory effectiveness against MERS-CoV.

conclusionIn this study, we first demonstrated that resveratrol is a potent anti-MERS agent in vitro. We perceive that resveratrol can be a potential antiviral agent against MERS-CoV infection in the near future.

Indexed as

AnimalsAntiviral AgentsChlorocebus aethiopsCoronavirus InfectionsMiddle East Respiratory Syndrome CoronavirusProtein BindingResveratrolRNA-Binding ProteinsRNA, ViralStilbenesVero CellsViral ProteinsAntiviral AgentsResveratrolRNA-Binding ProteinsRNA, ViralStilbenesViral ProteinsInhibitionMERS-CoVMERS-CoV virus infectionMiddle East Respiratory Syndrome VirusResveratrol

Identifiers

PMID28193191
PMCPMC5307780
OpenAlexW2588967599

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.