Evidence map›Paper›PMID 28192993›Full record

ArticleAnalytical chemistry2017

Quantitative Affinity Determination by Fluorescence Anisotropy Measurements of Individual Nanoliter Droplets.

Fabrice Gielen, Maren Butz, Eric J Rees, Miklos Erdelyi, Tommaso Moschetti, Marko Hyvönen, Joshua B Edel, Clemens F Kaminski, Florian Hollfelder

Abstract read
In one paragraph

Article in Analytical chemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Article
  2. Article
  3. Macromolecular High-Affinity Binding Probed by Advanced Fluorescence Techniques.Chembiochem : a European journal of chemical biology · 2025
    Review
  4. Article
  5. Review
  6. Article
  7. Article
  8. Improved RAD51 binders through motif shuffling based on the modularity of BRC repeats.Proceedings of the National Academy of Sciences of the United States of America · 2021
    Article
  9. Article
  10. Off-DNA DNA-Encoded Library Affinity Screening.ACS combinatorial science · 2020
    Article
  11. Activity-Based DNA-Encoded Library Screening.ACS combinatorial science · 2019
    Article
  12. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Fabrice GielenDepartment of Biochemistry, University of Cambridge , 80 Tennis Court Road, Cambridge, CB2 1GA, United Kingdom.
Maren ButzDepartment of Biochemistry, University of Cambridge , 80 Tennis Court Road, Cambridge, CB2 1GA, United Kingdom.
Eric J ReesDepartment of Chemical Engineering and Biotechnology, New Museums Site , Pembroke Street, Cambridge, CB2 3RA, United Kingdom.
Miklos ErdelyiDepartment of Chemical Engineering and Biotechnology, New Museums Site , Pembroke Street, Cambridge, CB2 3RA, United Kingdom.
Tommaso MoschettiDepartment of Biochemistry, University of Cambridge , 80 Tennis Court Road, Cambridge, CB2 1GA, United Kingdom.
Marko HyvönenDepartment of Biochemistry, University of Cambridge , 80 Tennis Court Road, Cambridge, CB2 1GA, United Kingdom.
Joshua B EdelDepartment of Chemistry, Imperial College London , South Kensington, London, SW7 2AZ, United Kingdom.
Clemens F KaminskiDepartment of Chemical Engineering and Biotechnology, New Museums Site , Pembroke Street, Cambridge, CB2 3RA, United Kingdom.
Florian HollfelderDepartment of Biochemistry, University of Cambridge , 80 Tennis Court Road, Cambridge, CB2 1GA, United Kingdom.ORCID 0000-0002-1367-6312

Funding

Medical Research Council MR/K02292X/1Wellcome Trust
6 · The paper itself

Abstract

Fluorescence anisotropy measurements of reagents compartmentalized into individual nanoliter droplets are shown to yield high-resolution binding curves from which precise dissociation constants (K

Identifiers

PMID28192993
PMCPMC5287478

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.