ArticleMethods and applications in fluorescence2016
Single molecule optical measurements of orientation and rotations of biological macromolecules.
Article in Methods and applications in fluorescence, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 39 citations in OpenAlex.
- Fused deep learning enables 6D single-molecule localization in polarization-resolved microscopy.Methods and applications in fluorescence · 2026Article
- Heterogeneity and multi-scale dynamics in the molecular bearing of the bacterial flagellum.Nature communications · 2026Article
- Torque-generating units of the bacterial flagellar motor are rotary motors.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Illuminating cellular architecture and dynamics with fluorescence polarization microscopy.Journal of cell science · 2024Review
- POLCAM: instant molecular orientation microscopy for the life sciences.Nature methods · 2024Article
- A new class of biological ion-driven rotary molecular motors with 5:2 symmetry.Frontiers in microbiology · 2022Review
- Membrane protein channels equipped with a cleavable linker for inducing catalysis inside nanocompartments.Journal of materials chemistry. B · 2021Article
- Single Molecules Are Your Quanta: A Bottom-Up Approach toward Multidimensional Super-resolution Microscopy.ACS nano · 2021Review
- Elucidating the Role of Topological Constraint on the Structure of Overstretched DNA Using Fluorescence Polarization Microscopy.The journal of physical chemistry. B · 2021Article
- No hype in hyperspace.Biophysical journal · 2021Article
- Measuring 3D orientation of nanocrystals via polarized luminescence of rare-earth dopants.Nature communications · 2021Article
- Advances of super-resolution fluorescence polarization microscopy and its applications in life sciences.Computational and structural biotechnology journal · 2020Review
- Super-resolution imaging of fluorescent dipoles via polarized structured illumination microscopy.Nature communications · 2019Article
- Single-molecule polarization microscopy of DNA intercalators sheds light on the structure of S-DNA.Science advances · 2019Article
- Nanoaperture fabrication via colloidal lithography for single molecule fluorescence analysis.PloS one · 2019Article
- Fluorescence anisotropy imaging in drug discovery.Advanced drug delivery reviewsReview
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Subdomains of macromolecules often undergo large orientation changes during their catalytic cycles that are essential for their activity. Tracking these rearrangements in real time opens a powerful window into the link between protein structure and functional output. Site-specific labeling of individual molecules with polarized optical probes and measurement of their spatial orientation can give insight into the crucial conformational changes, dynamics, and fluctuations of macromolecules. Here we describe the range of single molecule optical technologies that can extract orientation information from these probes, review the relevant types of probes and labeling techniques, and highlight the advantages and disadvantages of these technologies for addressing specific inquiries.
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