ArticleNature biotechnology2017
Dynamic regulation of metabolic flux in engineered bacteria using a pathway-independent quorum-sensing circuit.
Article in Nature biotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 179 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
179 citing papers in PubMed, 488 citations in OpenAlex.
- Quorum sensing-driven riboflavin-hyperproducing electroactive bacteria for enhanced bioelectricity generation from sludge: From modular optimization to process performance.Synthetic and systems biotechnology · 2027Article
- Challenges and Future Directives of Synthetic Biology in Engineering Plant-Microbe Partnerships for Sustainable Agriculture.Biotechnology and bioengineering · 2026Review
- Sustainable production of glutaric acid in microbial cell factories: Current advances and future prospects.Synthetic and systems biotechnology · 2026Review
- Construction of a pathway-independent three-module self-induced regulatory system and its application in 3-hydroxypropionic acid production.Engineering microbiology · 2026Article
- Metabolic engineering ofApplied and environmental microbiology · 2026Review
- Engineered Optogenetic Circuits In Yeast with Self-Sustained Outputs.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- An Experimentally Verified Mechanistic Model for Predicting Quorum Sensing-Based Switches.Microbial biotechnology · 2026Article
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- Scalable secondary metabolite production in Streptomyces using a plug-and-play system.Nature biotechnology · 2026Article
- Review
- Rethinking Microbial Chemical Ecology: Secondary Metabolites as Concentration-Dependent Signaling Hubs with Implications for Anti-Virulence Intervention.Microorganisms · 2026Review
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Advances in natural product discovery: strategies, technologies, and insights.Natural products and bioprospecting · 2026Review
- groovDB in 2026: a community-editable database of small molecule biosensors.Nucleic acids research · 2026Article
- Comprehensive genomic and metabolomic profiling ofFrontiers in microbiology · 2026Article
- Molecular adaptations and engineering of extremophiles for synthetic biology and biotechnological applications.Frontiers in microbiology · 2026Review
- DNA Origami and Its Applications in Synthetic Biology.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- EngineeringFrontiers in microbiology · 2026Review
- A stationary phase-specific bacterial green light sensor for enhancing metabolite production.Nature communications · 2025Article
- Balancing Cell Growth and Product Synthesis for Efficient Microbial Cell Factories.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
119 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Metabolic engineering of microorganisms to produce desirable products on an industrial scale can result in unbalanced cellular metabolic networks that reduce productivity and yield. Metabolic fluxes can be rebalanced using dynamic pathway regulation, but few broadly applicable tools are available to achieve this. We present a pathway-independent genetic control module that can be used to dynamically regulate the expression of target genes. We apply our module to identify the optimal point to redirect glycolytic flux into heterologous engineered pathways in Escherichia coli, resulting in titers of myo-inositol increased 5.5-fold and titers of glucaric acid increased from unmeasurable to >0.8 g/L, compared to the parent strains lacking dynamic flux control. Scaled-up production of these strains in benchtop bioreactors resulted in almost ten- and fivefold increases in specific titers of myo-inositol and glucaric acid, respectively. We also used our module to control flux into aromatic amino acid biosynthesis to increase titers of shikimate in E. coli from unmeasurable to >100 mg/L.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.