Evidence map›Paper›PMID 28191781›Full record

ArticleStem cells translational medicine2017

Direct Isolation and Characterization of Human Nephron Progenitors.

Stefano Da Sacco, Matthew E Thornton, Astgik Petrosyan, Maria Lavarreda-Pearce, Sargis Sedrakyan, Brendan H Grubbs, Roger E De Filippo, Laura Perin

Open access · goldAbstract readComparative StudyVideo-Audio Media
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells translational medicine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
3.0field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 25 citations in OpenAlex.

  1. Review
  2. Identification and Characterization of the Wilms Tumor Cancer Stem Cell.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023
    Article
  3. Article
  4. Nucleic-Acid Structures as Intracellular Probes for Live Cells.Advanced materials (Deerfield Beach, Fla.) · 2020
    Review
  5. Review
  6. Article
  7. Growing a new human kidney.Kidney international · 2019
    Review
  8. Article
  9. Article
  10. Article
  11. Review
  12. Review
  13. Article
  14. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Stefano Da SaccoGOFARR Laboratory for Organ Regenerative Research and Cell Therapeutics, Saban Research Institute, Division of Urology, Children's Hospital Los Angeles, Los Angeles, California, USA.
Matthew E ThorntonMaternal-Fetal Medicine Division, Department of Obstetrics and Gynecology, Keck School of Medicine, University of Southern California, Los Angeles, California, USA.
Astgik PetrosyanGOFARR Laboratory for Organ Regenerative Research and Cell Therapeutics, Saban Research Institute, Division of Urology, Children's Hospital Los Angeles, Los Angeles, California, USA.
Maria Lavarreda-PearceGOFARR Laboratory for Organ Regenerative Research and Cell Therapeutics, Saban Research Institute, Division of Urology, Children's Hospital Los Angeles, Los Angeles, California, USA.
Sargis SedrakyanGOFARR Laboratory for Organ Regenerative Research and Cell Therapeutics, Saban Research Institute, Division of Urology, Children's Hospital Los Angeles, Los Angeles, California, USA.
Brendan H GrubbsMaternal-Fetal Medicine Division, Department of Obstetrics and Gynecology, Keck School of Medicine, University of Southern California, Los Angeles, California, USA.
Roger E De FilippoGOFARR Laboratory for Organ Regenerative Research and Cell Therapeutics, Saban Research Institute, Division of Urology, Children's Hospital Los Angeles, Los Angeles, California, USA.
Laura PerinGOFARR Laboratory for Organ Regenerative Research and Cell Therapeutics, Saban Research Institute, Division of Urology, Children's Hospital Los Angeles, Los Angeles, California, USA.
Children's Hospital of Los Angeles · USUniversity of Southern California · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mature nephrons originate from a small population of uninduced nephrogenic progenitor cells (NPs) within the cap mesenchyme. These cells are characterized by the coexpression of SIX2 and CITED1. Many studies on mouse models as well as on human pluripotent stem cells have advanced our knowledge of NPs, but very little is known about this population in humans, since it is exhausted before birth and strategies for its direct isolation are still limited. Here we report an efficient protocol for direct isolation of human NPs without genetic manipulation or stepwise induction procedures. With the use of RNA-labeling probes, we isolated SIX2

Indexed as

AnimalsApoptosis Regulatory ProteinsCells, CulturedCell SeparationGene Expression Regulation, DevelopmentalHomeodomain ProteinsHumansMiceMorphogenesisNephronsNerve Tissue ProteinsNuclear ProteinsPhenotypeStem CellsTime FactorsTrans-ActivatorsApoptosis Regulatory ProteinsCITED1 protein, humanHomeodomain ProteinsNerve Tissue ProteinsNuclear ProteinsSIX2 protein, humanTrans-ActivatorsTranscription FactorsDirect isolation of live cellsHuman nephron progenitorsSmartflares

Identifiers

PMID28191781
PMCPMC5442819
OpenAlexW2509690144

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.