Evidence map›Paper›PMID 28190213›Full record

ArticleJournal of assisted reproduction and genetics2017

Quantitative and qualitative changes of mitochondria in human preimplantation embryos.

Shu Hashimoto, Naoharu Morimoto, Masaya Yamanaka, Hiroshi Matsumoto, Takayuki Yamochi, Hiroya Goto, Masayasu Inoue, Yoshiharu Nakaoka, Hiroaki Shibahara, Yoshiharu Morimoto

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of assisted reproduction and genetics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.

0numbers the graph read from it
0cells of the map it votes in
56citing papers in PubMed
9.4field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

56 citing papers in PubMed, 93 citations in OpenAlex.

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  10. Telomere Reprogramming and Cellular Metabolism: Is There a Link?International journal of molecular sciences · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Shu HashimotoIVF Namba Clinic, 1-17-28 Minamihorie, Nishi-ku, Osaka, 550-0015, Japan. hashimoto@ivfnamba.com.ORCID http://orcid.org/0000-0001-9083-1403
Naoharu MorimotoDepartment of Obstetrics and Gynecology, Hyogo College of Medicine, Nishinomiya, Hyogo, 663-8501, Japan.
Masaya YamanakaIVF Namba Clinic, 1-17-28 Minamihorie, Nishi-ku, Osaka, 550-0015, Japan.
Hiroshi MatsumotoIVF Namba Clinic, 1-17-28 Minamihorie, Nishi-ku, Osaka, 550-0015, Japan.
Takayuki YamochiIVF Namba Clinic, 1-17-28 Minamihorie, Nishi-ku, Osaka, 550-0015, Japan.
Hiroya GotoIVF Namba Clinic, 1-17-28 Minamihorie, Nishi-ku, Osaka, 550-0015, Japan.
Masayasu InoueIVF Namba Clinic, 1-17-28 Minamihorie, Nishi-ku, Osaka, 550-0015, Japan.
Yoshiharu NakaokaIVF Namba Clinic, 1-17-28 Minamihorie, Nishi-ku, Osaka, 550-0015, Japan.
Hiroaki ShibaharaDepartment of Obstetrics and Gynecology, Hyogo College of Medicine, Nishinomiya, Hyogo, 663-8501, Japan.
Yoshiharu MorimotoHORAC Grandfront Osaka Clinic, Osaka, 530-0011, Japan.
Hyogo Medical University · JPOsaka Nishi Clinic · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeThe oxygen consumption rates (OCRs) in mice and cattle have been reported to change during preimplantation embryogenesis. On the other hand, mitochondrial DNA (mtDNA) copy number has been shown to be unchanged in mice and changed in cattle and pigs. The interactions between mitochondrial functions and mtDNA copy numbers in human embryos during preimplantation development remain obscure.

methodsSixteen oocytes and 100 embryos were used to assess mtDNA copy numbers and OCR. Three oocytes and 12 embryos were used to determine cytochrome c oxidase activity. All specimens were obtained between July 2004 and November 2014, and donated from couples after they had given informed consent. Mature oocytes and embryos at 2-14-cell, morula, and blastocyst stages were used to assess their OCR in the presence or absence of mitotoxins. The mtDNA copy number was determined using the samples after analysis of OCR. The relationships between developmental stages and OCR, and developmental stages and mtDNA copy number were analyzed. Furthermore, cytochrome c oxidase activity was determined in oocytes and 4-cell to blastocyst stage embryos.

resultsThe structure of inner mitochondrial membranes and their respiratory function developed with embryonic growth and the mtDNA copy numbers decreased transiently compared with those of oocytes. The undifferentiated state of inner cell mass cells appears to be associated with a low OCR. On the other hand, the mtDNA copy numbers increased and aerobic metabolism of mitochondria increased in trophectoderm cells.

conclusionsThe mitochondrial respiratory function of human embryos developed along with embryonic growth although the copy numbers of mtDNA decreased transiently before blastulation. OCRs increased toward the morula stage ahead of an increase of mtDNA at the time of blastulation. Data regarding changes in mitochondrial function and mtDNA copy number during preimplantation development of human embryos will be useful for the development of ideal culture media.

Indexed as

BlastocystDNA Copy Number VariationsDNA, MitochondrialEmbryonic DevelopmentFemaleHumansMitochondriaMorulaOocytesOogenesisPregnancyDNA, MitochondrialMitochondrial DNAMitochondrial functionOxygen consumption

Identifiers

PMID28190213
PMCPMC5427646
OpenAlexW2587502116

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.