ArticleParasitology research2017
Update on Acanthamoeba jacobsi genotype T15, including full-length 18S rDNA molecular phylogeny.
Article in Parasitology research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Genotype distribution of Acanthamoeba in keratitis: a systematic review.Parasitology research · 2021Pooled it
- The pathogenesis, risk factors, diagnosis and treatment of Acanthamoeba keratitis.Frontiers in medicine · 2025Review
- Article
- First Report of Isolation and Characterization of Acanthamoeba spp. from the Milk Used for Calf Feeding.Acta parasitologica · 2024Article
- Proteases of Acanthamoeba.Parasitology research · 2023Review
- Sublethal Exposure to Plasma-Activated Water Influences the Morphological Characteristics, Phagocytic Ability, and Virulence of Acanthamoeba castellanii.Acta parasitologica · 2023Article
- Exposure to sublethal concentrations of chlorine enhances the cytotoxicity of Acanthamoeba castellanii.Parasitology research · 2023Article
- Biological characteristics and pathogenicity ofFrontiers in microbiology · 2023Review
- Real-Time PCR for the Diagnosis ofMicroorganisms · 2022Article
- Efficient nested-PCR-based method development for detection and genotype identification of Acanthamoeba from a small volume of aquatic environmental sample.Scientific reports · 2021Article
- Analysis of Acanthamoeba genotypes from public freshwater sources in Thailand reveals a new genotype, T23 Acanthamoeba bangkokensis sp. nov.Scientific reports · 2021Article
- Detection of Free-Living Amoebae and Their Intracellular Bacteria in Borehole Water before and after a Ceramic Pot Filter Point-of-Use Intervention in Rural Communities in South Africa.International journal of environmental research and public health · 2021Article
- The biology of Acanthamoeba keratitis.Experimental eye research · 2021Review
- Update on Acanthamoeba phylogeny.Parasitology research · 2020Article
- Isolation and identification of free-living amoeba from the hot springs and beaches of the Caspian Sea.Parasite epidemiology and control · 2020Article
- Species, Sequence Types and Alleles: Dissecting Genetic Variation inPathogens (Basel, Switzerland) · 2020Review
- Cartography of Free-Living Amoebae in Soil in Guadeloupe (French West Indies) Using DNA Metabarcoding.Pathogens (Basel, Switzerland) · 2020Article
- Free-living amoebae and their relationship to air quality in hospital environments: characterization ofParasitology · 2020Article
- Putative group I introns in the eukaryote nuclear internal transcribed spacers.Current genetics · 2020Article
- 18S rDNA sequencing aided diagnosis ofIndian journal of ophthalmology · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Free-living amoebae of the genus Acanthamoeba are worldwide present in natural and artificial environments, and are also clinically important, as causative agents of diseases in humans and other animals. Acanthamoeba comprises several species, historically assigned to one of the three groups based on their cyst morphology, but presently recognized as at least 20 genotypes (T1-T20) on the basis of their nuclear 18S ribosomal RNA (rRNA) gene (18S rDNA) sequences. While strain identification may usually be achieved targeting short (<500 bp) 18S ribosomal DNA (rDNA) fragments, the use of full-length gene sequences (>2200 bp) is necessary for correct genotype description and reliable molecular phylogenetic inference. The genotype T15, corresponding to Acanthamoeba jacobsi, is the only genotype described on the basis of partial sequences (~1500 bp). While this feature does not prevent the correct identification of the strains, having only partial sequences renders the genotype T15 not completely defined and may furthermore affect its position in the Acanthamoeba molecular tree. Here, we complete this gap, by obtaining full-length 18S rDNA sequences from eight A. jacobsi strains, genotype T15. Morphologies and physiological features of isolated strains are reported. Molecular phylogeny based on full 18S rDNA confirms some previous suggestions for a genetic link between T15 and T13, T16, and T19, with T19 as sister-group to T15.
Indexed as
Identifiers
28190156What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.