ArticleJournal of visualized experiments : JoVE2017
Preparation of Giant Vesicles Encapsulating Microspheres by Centrifugation of a Water-in-oil Emulsion.
Article in Journal of visualized experiments : JoVE, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Cell-free protein synthesis and vesicle systems for programmable therapeutic manufacturing and delivery.Journal of biological engineering · 2025Review
- Artificial cells and biomimicry cells: A rising star in the fight against cancer.Materials today. Bio · 2025Review
- Liposome-EncapsulatedACS synthetic biology · 2025Article
- Role of Shape in Particle-Lipid Membrane Interactions: From Surfing to Full Engulfment.ACS nano · 2024Article
- Alkyl anchor-modified artificial viral capsid budding outside-to-inside and inside-to-outside giant vesicles.Science and technology of advanced materials · 2024Article
- Quantitative Acoustophoresis.ACS nanoscience Au · 2022Article
- Thermo-Statistical Effects of Inclusions on Vesicles: Division into Multispheres and Polyhedral Deformation.Membranes · 2022Review
- Article
- On-Chip Inverted Emulsion Method for Fast Giant Vesicle Production, Handling, and Analysis.Micromachines · 2020Article
- Optimization of the Inverted Emulsion Method for High-Yield Production of Biomimetic Giant Unilamellar Vesicles.Chembiochem : a European journal of chemical biology · 2019Article
- Creation of Artificial Cell-Like Structures Promoted by Microfluidics Technologies.Micromachines · 2019Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The constructive biology and the synthetic biology approach to creating artificial life involve the bottom-up assembly of biological or nonbiological materials. Such approaches have received considerable attention in research on the boundary between living and nonliving matter and have been used to construct artificial cells over the past two decades. In particular, Giant Vesicles (GVs) have often been used as artificial cell membranes. In this paper, we describe the preparation of GVs encapsulating highly packed microspheres as a model of cells containing highly condensed biomolecules. The GVs were prepared by means of a simple water-in-oil emulsion centrifugation method. Specifically, a homogenizer was used to emulsify an aqueous solution containing the materials to be encapsulated and an oil containing dissolved phospholipids, and the resulting emulsion was layered carefully on the surface of another aqueous solution. The layered system was then centrifuged to generate the GVs. This powerful method was used to encapsulate materials ranging from small molecules to microspheres.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.