ArticleJournal of visualized experiments : JoVE2017
Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel.
Article in Journal of visualized experiments : JoVE, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed.
- The Rapid Anatomics Tool (RAT): A low-cost root anatomical phenotyping platform reveals changes in root anatomy along the root axis.Plant phenomics (Washington, D.C.) · 2026Article
- A long term time lapse microscopy technique for Arabidopsis roots.Frontiers in plant science · 2025Article
- SPIM-Flow: An Integrated Light Sheet and Microfluidics Platform for Hydrodynamic Studies ofBiology · 2023Article
- The cellulose synthase-like F3 (CslF3) gene mediates cell wall polysaccharide synthesis and affects root growth and differentiation in barley.The Plant journal : for cell and molecular biology · 2022Article
- Microscopic Imaging Methods for Organ-on-a-Chip Platforms.Micromachines · 2022Review
- Auxin-Regulated Lateral Root Organogenesis.Cold Spring Harbor perspectives in biology · 2021Review
- Probing the 3D architecture of the plant nucleus with microscopy approaches: challenges and solutions.Nucleus (Austin, Tex.) · 2019Review
- Non-canonical auxin signalling: fast and curious.Journal of experimental botany · 2019Article
- Global analysis of an exponential model of cell proliferation for estimation of cell cycle duration in the root apical meristem of angiosperms.Annals of botany · 2018Article
- Advances in Imaging Plant Cell Dynamics.Plant physiology · 2018Review
- What Has Been Seen Cannot Be Unseen-Detecting Auxin In Vivo.International journal of molecular sciences · 2017Review
- Article
- A Simple Chamber for Long-term Confocal Imaging of Root and Hypocotyl Development.Journal of visualized experiments : JoVE · 2017Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
One of the key questions in understanding plant development is how single cells behave in a larger context of the tissue. Therefore, it requires the observation of the whole organ with a high spatial- as well as temporal resolution over prolonged periods of time, which may cause photo-toxic effects. This protocol shows a plant sample preparation method for light-sheet microscopy, which is characterized by mounting the plant vertically on the surface of a gel. The plant is mounted in such a way that the roots are submerged in a liquid medium while the leaves remain in the air. In order to ensure photosynthetic activity of the plant, a custom-made lighting system illuminates the leaves. To keep the roots in darkness the water surface is covered with sheets of black plastic foil. This method allows long-term imaging of plant organ development in standardized conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.