ArticleJournal of visualized experiments : JoVE2017
Scanning Electron Microscopy (SEM) Protocols for Problematic Plant, Oomycete, and Fungal Samples.
Article in Journal of visualized experiments : JoVE, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 18 citations in OpenAlex.
- Single-phase silver nanoparticles from white tea: colloidal quality, mass-specific potency and bacterial envelope permeabilisation.RSC advances · 2026Article
- Culture Conditions Shape the Phenotype and Quality ofJournal of fungi (Basel, Switzerland) · 2026Article
- Rice Straw Tissue Preparation for Reproducible Electron Microscopy Imaging and Analysis.Current protocols · 2025Article
- Application and Mechanism of Action of Carvacrol AgainstJournal of fungi (Basel, Switzerland) · 2025Article
- Nano Emulsion of Essential Oils Loaded in Chitosan Coating for Controlling Anthracnose in Tomatoes (Foods (Basel, Switzerland) · 2025Article
- Scanning Electron Microscopy Reveals New Ultrastructural Features in Metacercariae ofPathogens (Basel, Switzerland) · 2025Article
- Influence of Drying Methods on the Morphological Features, Microstructural Properties, and Antioxidant Performance ofJournal of fungi (Basel, Switzerland) · 2025Article
- Natural variations of HvSRN1 modulate the spike rachis node number in barley.Plant communications · 2024Article
- Antifungal activity of indolicidin-derived peptide In-58 againstFrontiers in medicine · 2024Article
- InvestigatingAccess microbiology · 2023Article
- Physiological characterization of the wild almondFrontiers in plant science · 2022Article
- Integrated Physiological and Transcriptomic Analyses Responses to Altitude Stress in Oat (Frontiers in genetics · 2021Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Common problems in the processing of biological samples for observations with the scanning electron microscope (SEM) include cell collapse, treatment of samples from wet microenvironments and cell destruction. Using young floral tissues, oomycete cysts, and fungi spores (Agaricales) as examples, specific protocols to process delicate samples are described here that overcome some of the main challenges in sample treatment for image capture under the SEM. Floral meristems fixed with FAA (Formalin-Acetic-Alcohol) and processed with the Critical Point Dryer (CPD) did not display collapsed cellular walls or distorted organs. These results are crucial for the reconstruction of floral development. A similar CPD-based treatment of samples from wet microenvironments, such as the glutaraldehyde-fixed oomycete cysts, is optimal to test the differential growth of diagnostic characteristics (e.g., the cyst spines) on different types of substrates. Destruction of nurse cells attached to fungi spores was avoided after rehydration, dehydration, and the CPD treatment, an important step for further functional studies of these cells. The protocols detailed here represent low-cost and rapid alternatives for the acquisition of good-quality images to reconstruct growth processes and to study diagnostic characteristics.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.