Evidence map›Paper›PMID 28189871›Full record

ArticleBiomaterials2017

Vacuum-assisted decellularization: an accelerated protocol to generate tissue-engineered human tracheal scaffolds.

Colin R Butler, Robert E Hynds, Claire Crowley, Kate H C Gowers, Leanne Partington, Nicholas J Hamilton, Carla Carvalho, Manuela Platé, Edward R Samuel, Alan J Burns and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
42citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

42 citing papers in PubMed, 1 synthesis or guideline pooled it.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Colin R ButlerLungs for Living Research Centre, UCL Respiratory, University College London, London, UK; Stem Cell and Regenerative Medicine Section, UCL Institute of Child Health and Great Ormond Street Hospital, London, UK.
Robert E HyndsLungs for Living Research Centre, UCL Respiratory, University College London, London, UK.
Claire CrowleyStem Cell and Regenerative Medicine Section, UCL Institute of Child Health and Great Ormond Street Hospital, London, UK.
Kate H C GowersLungs for Living Research Centre, UCL Respiratory, University College London, London, UK.
Leanne PartingtonDepartment of Haematology, Royal Free Hospital and University College London, London, UK.
Nicholas J HamiltonLungs for Living Research Centre, UCL Respiratory, University College London, London, UK.
Carla CarvalhoDepartment of Haematology, Royal Free Hospital and University College London, London, UK.
Manuela PlatéLungs for Living Research Centre, UCL Respiratory, University College London, London, UK.
Edward R SamuelStem Cell and Regenerative Medicine Section, UCL Institute of Child Health and Great Ormond Street Hospital, London, UK.
Alan J BurnsStem Cell and Regenerative Medicine Section, UCL Institute of Child Health and Great Ormond Street Hospital, London, UK; Department of Clinical Genetics, Erasmus MC, Rotterdam, Netherlands.
Luca UrbaniStem Cell and Regenerative Medicine Section, UCL Institute of Child Health and Great Ormond Street Hospital, London, UK.
Martin A BirchallUCL Ear Institute, The Royal National Throat Nose and Ear Hospital, London, UK.
Mark W LowdellDepartment of Haematology, Royal Free Hospital and University College London, London, UK.
Paolo De CoppiStem Cell and Regenerative Medicine Section, UCL Institute of Child Health and Great Ormond Street Hospital, London, UK. Electronic address: p.decoppi@ucl.ac.uk.
Sam M JanesLungs for Living Research Centre, UCL Respiratory, University College London, London, UK. Electronic address: s.janes@ucl.ac.uk.

Funding

Department of Health PB-PG-1013-32058Department of Health RP_2014-04-046Medical Research Council G1001539Medical Research Council MR/K026453/1Medical Research Council MR/L002000/1Wellcome Trust WT107963AIA
6 · The paper itself

Abstract

Patients with large tracheal lesions unsuitable for conventional endoscopic or open operations may require a tracheal replacement but there is no present consensus of how this may be achieved. Tissue engineering using decellularized or synthetic tracheal scaffolds offers a new avenue for airway reconstruction. Decellularized human donor tracheal scaffolds have been applied in compassionate-use clinical cases but naturally derived extracellular matrix (ECM) scaffolds demand lengthy preparation times. Here, we compare a clinically applied detergent-enzymatic method (DEM) with an accelerated vacuum-assisted decellularization (VAD) protocol. We examined the histological appearance, DNA content and extracellular matrix composition of human donor tracheae decellularized using these techniques. Further, we performed scanning electron microscopy (SEM) and biomechanical testing to analyze decellularization performance. To assess the biocompatibility of scaffolds generated using VAD, we seeded scaffolds with primary human airway epithelial cells in vitro and performed in vivo chick chorioallantoic membrane (CAM) and subcutaneous implantation assays. Both DEM and VAD protocols produced well-decellularized tracheal scaffolds with no adverse mechanical effects and scaffolds retained the capacity for in vitro and in vivo cellular integration. We conclude that the substantial reduction in time required to produce scaffolds using VAD compared to DEM (approximately 9 days vs. 3-8 weeks) does not compromise the quality of human tracheal scaffold generated. These findings might inform clinical decellularization techniques as VAD offers accelerated scaffold production and reduces the associated costs.

Indexed as

Tissue ScaffoldsCell FractionationCell-Free SystemCells, CulturedEquipment DesignEquipment Failure AnalysisExtracellular MatrixHumansTissue EngineeringTracheaVacuumEpithelial cellsTissue engineeringTissue scaffoldsTracheaTransplantation

Identifiers

PMID28189871
PMCPMC5332556

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.