ArticleJournal of microbiological methods2017
A real-time PCR assay for accurate quantification of the individual members of the Altered Schaedler Flora microbiota in gnotobiotic mice.
Article in Journal of microbiological methods, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed.
- Tryptophan degradation by intestinal Bacteroides induces anti-tumor immunity and limits melanoma growth.Cell reports. Medicine · 2026Article
- Dietary sulfur amino acids enhance anti-tumor immunity in colon cancer via an NKT cell-XCL1-cDC1 circuit.Immunity · 2026Article
- Article
- A defined microbiota mouse model forFrontiers in microbiology · 2026Article
- Restoring low-fiber diets-inducedCurrent research in food science · 2026Article
- Temporal changes in fecal swine microbiome primarily reflectFrontiers in veterinary science · 2025Article
- Layer segmented filamentous bacteria colonize and impact gut health of broiler chickens.mSphere · 2024Article
- Microbiota analysis in individuals with type two diabetes mellitus and end‑stage renal disease: A pilot study.Experimental and therapeutic medicine · 2024Article
- Fiber-deficient diet inhibits colitis through the regulation of the niche and metabolism of a gut pathobiont.Cell host & microbe · 2023Article
- Treatment with Ligilactobacillus murinus lowers blood pressure and intestinal permeability in spontaneously hypertensive rats.Scientific reports · 2023Article
- Sterility testing of germ-free mouse colonies.Frontiers in immunology · 2023Review
- Segmented filamentous bacteria-based treatment to elicit protection againstFrontiers in microbiology · 2023Article
- Resources to Facilitate Use of the Altered Schaedler Flora (ASF) Mouse Model to Study Microbiome Function.mSystems · 2022Article
- Characterization of interactions of dietary cholesterol with the murine and human gut microbiome.Nature microbiology · 2022Article
- A conserved Bacteroidetes antigen induces anti-inflammatory intestinal T lymphocytes.Science (New York, N.Y.) · 2022Article
- Experimental evaluation of ecological principles to understand and modulate the outcome of bacterial strain competition in gut microbiomes.The ISME journal · 2022Article
- Immunoregulatory Intestinal Microbiota and COVID-19 in Patients with Type Two Diabetes: A Double-Edged Sword.Viruses · 2022Review
- Modulation of Gut Microbiota byFoods (Basel, Switzerland) · 2021Article
- Colonization with Altered Schaedler Flora Impacts Leukocyte Adhesion in Mesenteric Ischemia-Reperfusion Injury.Microorganisms · 2021Article
- Bacteria Broadly-Resistant to Last Resort Antibiotics Detected in Commercial Chicken Farms.Microorganisms · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
Abstract
Changes in the gastrointestinal microbial community are frequently associated with chronic diseases such as Inflammatory Bowel Diseases. However, understanding the relationship of any individual taxon within the community to host physiology is made complex due to the diversity and individuality of the gut microbiota. Defined microbial communities such as the Altered Schaedler Flora (ASF) help alleviate the challenges of a diverse microbiota by allowing one to interrogate the relationship between individual bacterial species and host responses. An important aspect of studying these relationships with defined microbial communities is the ability to measure the population abundance and dynamics of each member. Herein, we describe the development of an improved ASF species-specific and sensitive real-time quantitative polymerase chain reaction (qPCR) for use with SYBR Green chemistry to accurately assess individual ASF member abundance. This approach targets hypervariable regions V1 through V3 of the 16S rRNA gene of each ASF taxon to enhance assay specificity. We demonstrate the reproducibility, sensitivity and application of this new method by quantifying each ASF bacterium in two inbred mouse lines. We also used it to assess changes in ASF member abundance before and after acute antibiotic perturbation of the community as well as in mice fed two different diets. Additionally, we describe a nested PCR assay for the detection of lowly abundant ASF members. Altogether, this improved qPCR method will facilitate gnotobiotic research involving the ASF community by allowing for reproducible quantification of its members under various physiological conditions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.