Evidence map›Paper›PMID 28189605›Full record

ArticleReproductive toxicology (Elmsford, N.Y.)2017

Transcriptomics analysis of early embryonic stem cell differentiation under osteoblast culture conditions: Applications for detection of developmental toxicity.

Xinrong Chen, Tao Han, J Edward Fisher, Wafa Harrouk, Melissa S Tassinari, Gwenn E Merry, Daniel Sloper, James C Fuscoe, Deborah K Hansen, Amy L Inselman

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Reproductive toxicology (Elmsford, N.Y.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Xinrong ChenDivision of Systems Biology, National Center for Toxicological Research, Food and Drug Administration, United States. Electronic address: xchen@cpsc.gov.
Tao HanDivision of Systems Biology, National Center for Toxicological Research, Food and Drug Administration, United States. Electronic address: Tao.Han@fda.hhs.gov.
J Edward FisherCenter for Drug Evaluation and Research, Food and Drug Administration, United States. Electronic address: Jedward.fisher@fda.hhs.gov.
Wafa HarroukCenter for Drug Evaluation and Research, Food and Drug Administration, United States. Electronic address: Wafa.Harrouk@fda.hhs.gov.
Melissa S TassinariCenter for Drug Evaluation and Research, Food and Drug Administration, United States. Electronic address: Melissa.Tassinari@fda.hhs.gov.
Gwenn E MerryDivision of Systems Biology, National Center for Toxicological Research, Food and Drug Administration, United States. Electronic address: Gwenn.Merry@fda.hhs.gov.
Daniel SloperDivision of Systems Biology, National Center for Toxicological Research, Food and Drug Administration, United States. Electronic address: Daniel.Sloper@fda.hhs.gov.
James C FuscoeDivision of Systems Biology, National Center for Toxicological Research, Food and Drug Administration, United States. Electronic address: James.Fuscoe@fda.hhs.gov.
Deborah K HansenDivision of Systems Biology, National Center for Toxicological Research, Food and Drug Administration, United States. Electronic address: deborah_hansen@sbcglobal.net.
Amy L InselmanDivision of Systems Biology, National Center for Toxicological Research, Food and Drug Administration, United States. Electronic address: Amy.Inselman@fda.hhs.gov.

Funding

Translational Research CoreP30DK020541 · NIDDK · ALBERT EINSTEIN COLLEGE OF MEDICINE, INC · PI STREAMSON C CHUA · 2015 to 2026
$27.7M
Intramural FDA HHS FD999999NIDDK NIH HHS P30 DK020541
6 · The paper itself

Abstract

The mouse embryonic stem cell test (mEST) is a promising in vitro assay for predicting developmental toxicity. In the current study, early differentiation of D3 mouse embryonic stem cells (mESCs) under osteoblast culture conditions and embryotoxicity of cadmium sulfate were examined. D3 mESCs were exposed to cadmium sulfate for 24, 48 or 72h, and whole genome transcriptional profiles were determined. The results indicate a track of differentiation was identified as mESCs differentiate. Biological processes that were associated with differentiation related genes included embryonic development and, specifically, skeletal system development. Cadmium sulfate inhibited mESC differentiation at all three time points. Functional pathway analysis indicated biological pathways affected included those related to skeletal development, renal and reproductive function. In summary, our results suggest that transcriptional profiles are a sensitive indicator of early mESC differentiation. Transcriptomics may improve the predictivity of the mEST by suggesting possible modes of action for tested chemicals.

Indexed as

AnimalsCadmium CompoundsCell DifferentiationCells, CulturedGene Expression ProfilingGene Expression Regulation, DevelopmentalMiceMouse Embryonic Stem CellsOsteoblastsSulfatesCadmium Compoundscadmium sulfateSulfatesEmbryonic stem cell testEmbryotoxicityOsteoblastTranscriptomics

Identifiers

PMID28189605
PMCPMC5427642

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.